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ONE STRAIN OF ENDOPHYTIC PREUSSIA, A POTENTIAL BIOLOGICAL PARTNER OF TOMATO SEEDLINGS AGAINST ALTERNARIOSIS


CRISTINA TANE 1, LAVINIA BARBU 1, SAMUEL RODRIGUEZ SABINA 2, DANIEL COJANU 1, ANDREEA COSOVEANU 1
1. Research-Development Institute for Plant Protection, Ion Ionescu de la Brad Bd., no. 8, CP 013813, Bucharest, Romania, Corresponding author: andreeacosoveanu@gmail.com;
2. Department Botanica, Ecologia & Fisiologia Vegetal, Universidad de La Laguna, 38206 La Laguna, Tenerife, Spain.

Issue:

SCSB, Number 1, Volume XXVIII

Section:

Volume 28, No. 1

Abstract:

A fungal endophytic strain, isolated from Artemisia sp., identified as Preussia sp. was selected for its potential of plant growth promotion and reduction of pathogen attack, in vitro.

The metaorganism tomato - endophytic fungus - fungal pathogen was observed in terms of germination, root and shoot weight, number of leaves and percentage of leaves pairs (1 – 3), index of development, index of necrosis and index of attack. The time sequence employed for the entrance of the partners in the setting was: moment 0 = endophyte, moment 1 = tomato seeds (after 2 days from M0), moment 2 = pathogen (after 14 days from M1) and moment 3 = data collection (after 7 days from M2). A positive influence on germination was observed when all three partners interacted, compared to all variables (all partners “T1” - 100% versus endophytic fungus and seed “C1”- 66.67%, pathogen and seed “C2” - 88.89, seed alone “C3”- 77.78%, respectively).

Root and shoot length was improved both when all partners “T1” (root – 7.30 cm, shoot – 4.58 cm) as well as when endophytic fungus and seed partners “C1” (root – 8.40 cm, shoot – 4 cm) were present, compared to seed alone “C3” (root – 7.70 cm and shoot – 2.73 cm) and seed and pathogen partners “C2” (root – 5.74 cm and shoot – 3.06 cm). Although the average number of pairs was similar among the variables (C2 - 1.5; C3 - 1.6; T1 and C1 - 1.7), the percentage of pairs of leaves differed significantly for the percentage of 1 pair of leaves: C3 = 42.9% versus T1 = 11.1%. An improvement was observed in the percentage of 2 pairs of leaves for the case of all partners as well as for the case of endophyte and seed: T1 = 77.78%, C1 = 85.7% versus C2 = 62.5% and C3 = 57.15. Yet, the index of development showed best case when seed was alone and no difference between pathogen or endophyte presence.

In terms of the indices of necrosis (IN) and attack (IA), the endophyte was innocuously interacting with seedling (IN and IA = 0) and also when both pathogen and endophyte (T1) were present the seedling presented no symptoms, compared to pathogen and seedling case (C2): IN - T1 = 0 versus C2 = 0.78 and IA – T1 = 0 versus C2 = 11.11.

Keywords:

biological control, mutualist fungi, Alternaria, phytopathogens.

Code [ID]:

SCSB201901V28S01A0002 [0005027]

Note:

Full paper:

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