EXTRACTION AND ESTIMATION OF CHLOROPHYLL CONTENT IN CABBAGE SEEDLING LEAVES (BRASSICA OLERACEA L. SILVIANA VARIETY) GROWN IN SOIL CONTAMINATED WITH DIFFERENT CONCENTRATIONS OF CADMIUM AND COPPER
GABRIEL-ALIN IOSOB 1, VALENTIN NEDEFF 2, MARIA PRISECARU 3, TINA OANA CRISTEA 4 1. Vegetable Research and Development Station Bacău, 220 Calea Bârladului Str., 600388 Bacău and Vasile Alecsandri University of Bacău, Faculty of Engineering, 157 Mărăşeşti Str., 600115 Bacău, România, e-mail: iosob.gabriel@gmail.com;
2. Vasile Alecsandri University of Bacău, Faculty of Engineering, 157 Mărăşeşti Str., 600115 Bacău, România and Gheorghe Ionescu Siseşti Academy of Agricultural and Forest Sciences, 61 Mărăşti Str., 011464 Bucharest, Romania, e-mail: vnedeff@ub.ro;
3. Vasile Alecsandri University of Bacău, Faculty of Biology, 157 Mărăşeşti Str., 600115 Bacău, România, e-mail: prisecaru_maria@yahoo.com;
4. Vegetable Research and Development Station Bacău, 220 Calea Bârladului Str., 600388 Bacău, România, e-mail: tinaoana@yahoo.com
Vegetables are essential in human diet and the presence of HM in excess it is a global issue that threaten plant health, wildlife and people [4]. This study was conducted under the protocol OECD 208. And values of Cd and Cu solutions were established in Order no. 756/3 November 1997 with regard to traces of chemical elements in the soil. Chlorophyll pigments evaluation was performed following the protocol of Lichtenthaler (1987) [11]. After data analysis the difference observed between the amount of Chl a and Chl b was very small in all the variants studied. The largest amount of total chlorophyll and the small amount of carotenoids it was determined variant tow (V2 = 100 mg Cu). And coefficient of variation for photosynthetic pigments is not greater than 0.45%.
