MORPHOGENETIC STUDIES CONCERNING THE MAIN HAPLOID PATHWAYS "IN VITRO" AT BRASSICA OLERACEA var. ITALICA L.
TINA OANA CRISTEA 1, MARIA CALIN 1, GABRIEL -ALIN IOSOB 2, NELLY FÂNARU 1, ANA MARIA NECHITA 1, MARIA PRISECARU 3 1. Vegetable Research and Development Station Bacau, Calea Barladului, No. 220, Bacau, code: 600388, e-mail: tinaoana@yahoo.com
2. Vegetable Research and Development Station Bacău, 220 Calea Bârladului Str., 600388 Bacău and Vasile Alecsandri University of Bacău, Faculty of Engineering, 157 Mărăşeşti Str., 600115 Bacău, România, e-mail: iosob.gabriel@gmail.com
3. „Vasile Alecsandri”, University of Bacau, Faculty of Science, Department of Biology, Marasesti Street, no. 157, Bacau, Romania, e-mail: prisecaru_maria@yahoo.com
The present paper aims at carrying out an integrative study to establish the most effective method of obtaining haploid plants in Brassica oleracea L. var. capitata, using "in vitro" culture, respectively androgenesis and non-fecunded ovary culture - ginogenesis, an alternative method of producing haploid plants, less mentioned in the literature at Brassica, but with which haploid plants and isogenic lines have been successfully obtained in other species. The study revealed the superiority of anther cultures in the initiation of cultures dedicatet to the production of haploid plants in Brassica oleracea L. The success of these cultures "in vitro" depends both on genotype and on culture conditions.
