ANALYTICAL PROBLEMS OF BILIRUBIN DETERMINATION BY DIAZOREACTION METHOD IN ICTERIC NEONATES IN ALGERIA
EL KADI FATIMA ZOHRA 1, BOUANANI FATIMA ZOHRA 2, KANOUN KHEDOUDJA 3, BOUARFA AMINE 4, MAAZ ASMA 4, SOFIANE BOUAZZA 2, MEZIANI SAMIRA 2 1. Process engineering Laboratory, materials and environment, Faculty of Technology, Djillali Liabés University of Sidi-Bel-Abbés, Algeria, e-mail: elkadifatimazohra@yahoo.fr
2. Biotoxicology Laboratory, Biology Department , Faculty of Natural Sciences and Life ,Djillali Liabés University of Sidi-Bel-Abbés, BP N°. 89 Sidi Bel Abbes 22000 Algeria
3. Molecular Microbiology Health and Proteomics Laboratory, Biology Department , Faculty of Natural Sciences and Life ,Djillali Liabés University of Sidi-Bel-Abbés, Algeria, e-mail: khedoudja.kanoun@univ-sba.dz and khedi20022002@yahoo.fr
4. Medical Analysis Central Laboratory of the Mother and Child of Sidi-Bel-Abbés Hospital
The determination of bilirubin remains the key examination to diagnose neonatal icterus and its severity and on which the therapeutic decision is based, The objective of our study is to detect the choice method for the bilirubin determination .To this effect, 100 cases of newborn babies with mucocutaneous icterus at the Neonatology Department of the maternity Hospital of the Sidi Bel Abbés region (Algeria) were subjected to bilirubin determination by diazoreaction manually by spectrophotometry using the ERBA and Biomaghreb reagents and automatically by means of an automaton using the ERBA reagent. Our results showed that the accelerating factor and the incubation time influence the most sensitive fraction of bilirubin which is the total bilirubin, Similarly, the cetrimide accelerant of the ERBA reagent is more efficient than the DMSO of the Biomaghreb reagent. In view of the advantages of the automatic technique and the quality of the reagent used, ERBA and its cetrimide booster, it is chosen as the reference technique for the bilirubin determination in neonates.
