Abstract
The determination of vitamin C has gained increased significance in several areas of analytical chemistry such as pharmaceutical, clinical and food application. In this paper, three rapid and accurate methods of determining ascorbic acid are presented. The proposed methods were successfully applied to pharmaceutical preparations. Potassium iodide has been used as the titrant for the determination of acid ascorbic, according to pharmacopoeias. The present paper reports on a direct spectrophotometric method of determining ascorbic acid with chloric acid 0.1 N by measuring the absorbance of the reaction at 245 nm. HPLC analyses of ascorbic acid on column Lichrosorb NH2 with acetonitril-KH2PO4 as the mobile phase were compared with the titrimetric and spectrophotometric methods.