CHARACTERIZATION OF A TEM CELL-BASED SETUP FOR THE EXPOSURE OF BIOLOGICAL CELL SUSPENSIONS TO HIGH-INTENSITY NANOSECOND PULSED ELECTRIC FIELDS (nsPEF)

  • SOUEID MALAK
    Institut de recherche XLIM, UMR CNRS 7252 Université de Limoges, 87060 Limoges, France
  • KOHLER SOPHIE
    Institut de recherche XLIM, UMR CNRS 7252 Université de Limoges, 87060 Limoges, France
  • O’CONNOR RODNEY P.
    Institut de recherche XLIM, UMR CNRS 7252 Université de Limoges, 87060 Limoges, France
  • LEVEQUE PHILIPPE
    Institut de recherche XLIM, UMR CNRS 7252 Université de Limoges, 87060 Limoges, France
  • ARNAUD-CORMOS DELIA
    Institut de recherche XLIM, UMR CNRS 7252 Université de Limoges, 87060 Limoges, France

Abstract

Cellular and intracellular structures of biological cells can be disturbed by exposure to nanosecond pulses electric field (nsPEF). The exposure system comprises two main parts: the pulses generator and the delivery device that containing biological medium. In this paper, we propose and characterize an open Transverse ElectroMagnetic (TEM) cell to expose a Petri dish filled with a biological suspension to nanosecond high voltage pulsed electric fields. Measurements of the reflection (S11) and transmission (S21) parameters show that empty TEM cell has a bandwidth of 2.9 GHz, whereas when the Petri dish is filled bandwidth is 1.7 GHz. TEM cell is well suited to deliver ultra-short pulsed electric fields with 1 ns duration.

Cuvinte cheie

Bioelectromagnetism Transverse ElectroMagnetic (TEM) cell S parameters pulsed electric fields high voltage.