In Europe, anaerobic digestion is a sector in full development. The solid product of this anaerobic digestion is used as a fertilizer. In terms of health, it is essential to ensure the safety of the digestate spread on agricultural land. Treatments upstream of anaerobic digestion could potentiate the hygenizing effect of anaerobic digestion. Acid and heat pretreatments were tested in this study. These resulted in more or less significant reductions in the concentration of Escherichia coli, enterococci and spores of Clostridium perfringens. The combination of different pre-treatments would be interesting to study in future research.