Our study aims to identify vibrational differences associated with the cell cycle by multiplex CARS micro spectroscopy in the CH zone. For this purpose, a human cell line HEK293 is used and blocked either in interphase or prophase and then analysed by CARS mapping on the vibration modes associated with proteins and lipids. We have shown that using the vibrational mode of proteins, we can characterize the state of chromatin condensation, moving from a low condensed state (interphase) to hypercondensed (mitosis). This hypercondensation is ensured by the binding of condensins to chromatin, proteins that are associated with chromatin to promote compaction.