BROADBAND COHERENT ANTI-STOKES RAMAN SCATTERING HIGHLIGHTS STATE OF CHROMATIN CONDENSATION IN THE HIGH WAVENUMBER.

  • Tiffany Guerenne-Del Ben
    1EA 7500 PEIRENE, </br> 2XLIM UMR 7252, University of Limoges, 123 avenue Albert Thomas, 87060 Limoges
  • Philippe Leproux
    1EA 7500 PEIRENE, </br> 2XLIM UMR 7252, University of Limoges, 123 avenue Albert Thomas, 87060 Limoges
  • Vincent Couderc
    1EA 7500 PEIRENE, </br> 2XLIM UMR 7252, University of Limoges, 123 avenue Albert Thomas, 87060 Limoges
  • Vincent Sol
    1EA 7500 PEIRENE, </br> 2XLIM UMR 7252, University of Limoges, 123 avenue Albert Thomas, 87060 Limoges
  • Jean-Michel Petit
    1EA 7500 PEIRENE, </br> 2XLIM UMR 7252, University of Limoges, 123 avenue Albert Thomas, 87060 Limoges

Abstract

Our study aims to identify vibrational differences associated with the cell cycle
by multiplex CARS micro spectroscopy in the CH zone. For this purpose, a human cell line
HEK293 is used and blocked either in interphase or prophase and then analysed by CARS
mapping on the vibration modes associated with proteins and lipids. We have shown that
using the vibrational mode of proteins, we can characterize the state of chromatin
condensation, moving from a low condensed state (interphase) to hypercondensed (mitosis).
This hypercondensation is ensured by the binding of condensins to chromatin, proteins that
are associated with chromatin to promote compaction.

Cuvinte cheie

Coherent Anti-Stokes Raman Scattering microspectroscopy Human cell line HEK293 High wavenumber spectral region state of chromatin condensation