The interest of multiphoton microscopy (MPM) is now well established in life sciences, especially thanks to its multispectral aspect. However, this property is limited by commercial laser systems currently involved in MPM, presenting a short spectral bandwidth. At best, four constituents can be imaged simultaneously. Here, we present an original spectral filtering system coupled with a large bandwidth laser system. The adaptation of this system to MPM in life sciences is demonstrated of target samples from plant or animal origin.