The article presents the features of inheritance of the main economically valuable traits of F1 tomato hybrids and the manifestation of heterosis effect on them in combinations from crossing mutant, semi-mutant forms with genotypes of cultural type. The ambiguous influence of mutant marker genes on the character of inheritance of traits and manifestation of heterosis effect on them is shown. The F1 hybrids (rin, nor, Ln, Wom) inherited components and subcomponents of productivity, total yield and fruit marketability with simultaneous manifestation of high heterosis effect on them by the type of positive and overdominance. The trait “duration of vegetation period” was inherited by the intermediate type. Negative dominance and overdominance of the main economically valuable traits are observed in combinations with sterility genes (ps-2 and ps), including pollen quality and its response to high and low temperatures. The highest heterosis effect on all studied traits is observed in combinations of intermutant F1 hybrids. F1 hybrids were identified: L186(rin) × Mo 835(Ln); L8/234(nor) × Mo 835(Ln); L201(nor) × Mo341(Wom) with stable high heterosis effect on productivity subcomponents, total yield, fruit marketability, with high adaptive potential to the action of high and low temperature stress factors in three different years of research (2020-2022).