The pathogenic microorganisms related to the UTIs etiology frequently belong to the Enterobacteriaceae family. This present study aims the following: to determine the Enterobacteriaceae isolated from the urinary cultures, displaying the most common species, such as: Escherichia coli, Klebsiella spp., and Proteus spp.; to test the biochemical and culture medium-related characteristics by means of some multi-test medium variants (TSI, MIU, Simmons Citrate) and of the API 20 E kit (Biomerieux) to identify the species; to correlate the microbiological data with the clinical factors. The survey on the distribution and number of UTIs from Moldavia region (Romania) during the analyzed period of time pointed out a rather good health state in the human population based on the pathogens’ frequency and the resistance patterns.