The article presents the research results the purpose of which was to evaluate the possibility of utilization of nutrient media obtained from carrot and celery agri-food wastes as a single source of nutrition for the cultivation of pigmented yeast strains of the genus Rhodotorula. It was established that nutrient media based on carrot and mixture of carrot and celery present an effective substrate for the cultivation of pigmented yeasts R. mucilaginosa CNMN-Ys-10 and R. gracilis CNMN-Ys-03, which allow stimulation of the biomass productivity of yeasts, significantly increase the concentration of carotenoids in biomass and increase the total antioxidant activity of the strain biomass. The medium based on celery wastes inhibits the growth and development of the strains, affects the yeast productivity, significantly modifies the biochemical composition of the biomass and significant increases its total antioxidant activity.