Vitamin C is an important biological molecule involved in many biological and biochemical processes as reducing agent, enzyme cofactor and nutritional factor. Due to the widespread use of vitamin C, a large number of methods have been developed for quantifying vitamin C contents in natural and fortified food samples and pharmaceuticals: titrimetry, voltammetry, potentiometry, fluorometry, spectrophotometry, kinetic-based chemiluminescence, flow injection analyses (FIA) and chromatography. It is therefore essential to assess these methods. Accordingly, this paper reviews spectrophotometric and chromatographic methods for the determination of vitamin C from food samples and pharmaceutical products.