THE STUDY OF “IN VITRO” MULTIPLICATION OF SUGAR BEET (BETA VULGARIS L.)
PRISECARU MARIA 1, GHEORGHIŢĂ GOGU 1, FÎNARU ADRIANA 2 (1) UNIVERSITATEA BACĂU, FACULTATEA DE LITERE SI STIINTE, CALEA MARASESTI 157, 5500 BACĂU, ROMÂNIA (2) UNIVERSITATEA BACĂU, FACULTATEA DE INGINERIE, CALEA MARASESTI 157, 5500 BACĂU, ROMÂNIA
By using the basal medium MS (Murashige - Skooge, 1962) supplemented with various concentrations of auxins and cytokinins, callus cultures were obtained from all the 19 genotypes tested by using cotyledons, hypocotyls, petioles and leaves as sources of explants. The best results were obtained on the medium with cytochinins (BAP, Kinetin) 5mg/L, associated with 2,4-D 0.1mg/L. The green, friable callus transferred on the regeneration medium (BAP 5mg/L, NAA 0.1mg/L, GA3 0.05mg/L,) developed buds and shoots. By transferring the shoots on the medium with IAA 2mg/L, and Kinetin 0.02mg/L, all shoots developed roots after approximately 3 weeks. The complete pants regenerated were successfully adapted to the septic conditions, both in the laboratory and in the greenhouse. Passing off explants directly on the regeneration medium showed their answering ability through direct organogenesis. The intensity of the direct and indirect organogenesis process was satisfactory, despite of nature of initial explants. The genotypes Pl6, Pl7 (diploid) and SG29 (tetraploid) excelled in producing a high yield of regenerates.