The aminoacids that are present in the
haploid plants of 10-12 cm length obtained by
gynogenesis âin vitroâ via the culture of nonfertilized ovaries and ovules have been identified
and quantitatively measured in wheat (Triticum
aestivum L.), sugarbeet (Beta vulgaris L.),
sunflower (Helianthus annuus L.) and potato
(Solanum tuberosum L.) by using the
chromatographic method upon thin larger and
UV-VIS spectrophotometry.
Diploid plants of the same length where
used for control. They were obtained by
germination of the donor plants seeds from where
ovaries and ovules for cultivation in vitro were
sampled. Thus, it is found that in the haploids
obtained in vitro, for all studied species, the
values of aminoacids are in general clearly low,
as compared to the control diploid plants.
The haploid plants chromatograms show
also the lack of some aminoacids identified in the
control diploid plants.
It is interesting to note that in some
haploids new aminoacids appear, but they are not
identified in the 2n plants (phenylalanine, in the
potato haploid), or in almost equal quantitative
values, or higher that the 2n donor plant value (
this in the case of methionine in wheat haploid
and histidine in sunflower haploid). |