This study aims to characterize the antimicrobial properties of copper coatings deposited by thermal spraying (plasma and wire-arc) on a wood substrate. The antimicrobial properties of these deposits were evaluated for different bacterial strains: E. coli, S. epidermis, E. faecalis, B. subtilis. The structural characterization of the deposits showed that they were continuous but revealed differences in their chemical composition. These observations were correlated with microbiological results. Deposits obtained after plasma spraying, without copper oxide have less important bactericidal properties.