Abstract: | Utilizing the âin vitroâ culture of unfertilized ovaries we succeeded on obtaining plants with gynogenetic origin via callus, at the potato variety DesirĂ©e (Solanum tuberosum L.). For the induction of gynogenesis the best hormonal combination was: BAP (0,3 mg/l), NAA (0,1 mg/l) and 2,4 D (0,05 mg/l). The basic media utilized in our experiments was MS (1962), supplemented with active charcoal (0,5 mg/l). The differentiation media with BAP (1-2 mg/l) associated with NAA or IAA (0,5 mg/l) generated embryos and plants.
The association of BAP or zeatin with GA3 (0,5 mg/l) determined the formation of a large number of micro-tubers both on plants and in the interior of media. The micro-tubers formation was stimulated also by a larger quantity of sucrose (8 %). The micro-tubers and fragments of plantlets represented a good material for âin vitroâ micropropagation. The plants obtained from the gynogenetic callsu were 56% diploid, 28% haploid, 3 plants aneuploid and one mixoploid. |