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BROADBAND COHERENT ANTI-STOKES RAMAN SCATTERING HIGHLIGHTS STATE OF CHROMATIN CONDENSATION IN THE HIGH WAVENUMBER.


TIFFANY GUERENNE-DEL BEN*1, PHILIPPE LEPROUX2, VINCENT COUDERC2, VINCENT SOL1, JEAN-MICHEL PETIT1
1EA 7500 PEIRENE,
2XLIM UMR 7252, University of Limoges, 123 avenue Albert Thomas, 87060 Limoges
*Tiffany.guerenne-del_ben@unilim.fr

Issue:

PLUMEE, Number 1, Volume IX

Section:

Issue No.1 - Volume 9 (2019)

Abstract:

Our study aims to identify vibrational differences associated with the cell cycle

by multiplex CARS micro spectroscopy in the CH zone. For this purpose, a human cell line

HEK293 is used and blocked either in interphase or prophase and then analysed by CARS

mapping on the vibration modes associated with proteins and lipids. We have shown that

using the vibrational mode of proteins, we can characterize the state of chromatin

condensation, moving from a low condensed state (interphase) to hypercondensed (mitosis).

This hypercondensation is ensured by the binding of condensins to chromatin, proteins that

are associated with chromatin to promote compaction.

Keywords:

Coherent Anti-Stokes Raman Scattering microspectroscopy, Human cell line HEK293, High wavenumber spectral region, state of chromatin condensation.

Code [ID]:

PLUMEE201901V09S01A0015 [0004983]

Note:

DOI:

Full paper:

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