The present paper presents a hydrophilic interaction chromatography (HILIC) coupled to tandem mass spectrometry method for the analysis of 11 catecholamines, precursors and metabolites in a sheep brain extract. The LODs were between 0.5 ngâmL-1 (3-MT) and 250 ngâmL-1 (DOPAC). The method accuracy has been evaluated in brain extract by recovery studies with samples spiked at a concentration of 100 ngâmL-1. For the spiked brain extract, the mean recovery values ranged from 81 % to 101 %, with precision < 8 %.