SYNTHESIS OF GIBBERELLINS BY SURFACTANT PRODUCERS NOCARDIA VACCINII IMV Đ-7405, ACINETOBACTER CALCOACETICUS IMV Đ-7241 AND RHODOCOCCUS ERYTHROPOLIS IMV ĐĐĄ-5017
NATALIA LEONOVA 1, TETYANA PIROG 1, 2, DARIA PIATETSKA 2*, TETYANA SHEVCHUK 1, MAXYM KHARKHOTA 1, GALYNA IUTYNSKA 1 1. D.K. Zabolotny Institute of Microbiology and Virology of National Academy of Science of Ukraine, 03143, Acad. Zabolotny str., 154, Kyiv, Ukraine
2. National University of Food Technologies, Faculty of Biotechnology and Ecological Control, 01601 Volodymyrska str., 68, Kyiv, Ukraine
*Corresponding author: dashka2310@gmail.com
In this study, we established that the producers of surfactants Nocardia vaccinii IMV Đ-7405, Acinetobacter calcoaceticus IMV B-7241 and Rhodococcus erythropolis IMV Đc-5017 were able to synthesize phytohormones of gibberellic nature during cultivation on the traditional substrates (ethanol, hexadecane) and on the industrial waste sunflower oil and biodiesel production waste). The results of specific biotesting showed that the treatment of cucumber seedlings with phytohormonal extracts of all studied strains at 1:500 and 1:600 dilutions stimulated the extension of the cucumber hypocotyls as compared to the water control in values close to the treatment with gibberellic acid. The results of high-performance liquid chromatography showed N. vaccinii IMV B-7405, A. calcoaceticus IMV B-7241 and R. erythropolis IMV Đc-5017 produce highly active forms of gibberellins GA3 and GA4. The level of its synthesis was nearly the same (6.0-10.0 ÎŒgâL-1) under cultivation of strains on every substrate. The exception was strain N. vaccinii IMV Đ-7405 which synthesized almost 47.0 ÎŒgâL-1 GA3 and GA4 while is growing on the waste oil from meat frying. The obtained results are the groundwork for the development of an economically profitable technology for the recycling of toxic wastes using N. vaccinii IMV B-7405, A. calcoaceticus IMV B-7241 and R. erythropolis IMV Đc-5017. Such technology will allow us to develop complex microbial preparations with various biological properties in a single process.
