Volume 29, No. 2 (2020)
Articles
ASSESMENT OF SEXUAL AND REPRODUCTIVE CORRELATION IN THE SEASONAL DYNAMICS OF THE AGROTIS SEGETUM POPULATION AT IMAGO STAGE
TUDOR NASTAS, IULIANA RUSU
During seasonal activity A. Segetum has been shown to evolve over three generations. The development of the first generation was noted during the first decade of May and the second decade of June. The development of the second and third generations was established between the first decade of July and the second decade of September. The assessment of the sexual correlation of imago Agrotis segetum during crop growing, showed the ratio of males to females is 1,3 : 0,8. The Agrotis segetum phytophagus has been shown that the present climate of the Republic of Moldova, increased aggressiveness on crops, and the number of eggs in the egg tubes of the females does not depend on the number of copulatory acts (estimated by the number of spermatophores in the copulatory bags of females). Analysis of the females showed that the copulative bags contained between 1 and 6 sperm, which means that they were paired with the males up to six times. Females with 2 pairs (30%) and 3 pairs (23%), representing 53% of the total number of captured females.
PRODUCTION OF THE LABORATORY HOST CEREAL MOTH (SITOTROGA CEREALELLA OL.) ON DIFFERENT CEREAL SUBSTRATES FOR THE TRICHOGRAMMA ENTOMOPHAGUS’ REARING
LIDIA GAVRILITA
In the variants with thermal processing, the quantity of eggs obtained on the substrates of barley, wheat, maize and triticale from 1 kg 7-8 grams were collected, in control, where the substrates were not processed this index varied respectively 6.0-6.5 grams. The percentage of infestation of the substrates with S. cerealella Ol. and the percentage of parasitation eggs of T. pintoi Voeg. and T. evanescens Westw., in the variants with thermal processing is higher, than in the control. The values of the barley infestation percentage and the parasitic percentage of T. pintoi and T. evanescens obtained on eggs of the barley substrate is slightly higher than on wheat, maize and triticale.
CYTOLOGICAL OBSERVATIONS ON CERVICAL CANCER LESIONS
MARIA PRISECARU(1), IONUŢ STOICA(1), TATIANA CIUREA(2), CRINA BOBREAZ(3), FLORIAN PRISECARU(4), GABRIEL ALIN IOSOB(5)
This paper aims to implement and understand the mechanisms by which cervical lesions intervene in the pathogenesis of lesions precursor to neoplasia, as well as knowledge of their cytological investigation methods. The study is channeled towards a complete evaluation of the complex diagnosis of cervical intraepithelial lesions, based on classical methods of investigation that include the Babeş-Papanicolaou cytological examination. The cervix can be easily examined cytologically. The use of this method of investigation leads to the discovery of the disease in the early stages, 100% curable (Koss, 1989). The observations were made on cervico-vaginal smears. The vaginal secretion product was collected from 282 patients examined in the obstetrics-gynecology office "Clinic Center Hera" Bacau, for one year, March 2019 - March 2020. The samples were processed and clinically examined in the Medlife laboratory, Bacau . The working method used was Papanicolau in liquid medium, May-Grünwald-Giemsa staining, Papanicolau classification. Following the study, 73.85% of patients were identified with PN I and II smears, 8.83% were patients with PN III smears and 6.36% were patients with PN IV. Most cytological changes in the cervix occur in sexually active women over the age of 35.
ANALYZING THE IMPORTANCE OF GAME AND FISH SPECIES FROM HARGHITA COUNTY
CĂTĂLIN IONEL CIONTU(1), LUCIAN DINCĂ(2)
The surface of game funds from Harghita County amounts to 593.944 ha gathered in 45 funds. They are characterized by a moderately continental climate in Sub-Carpathian hills and a mountain climate in the high areas. Eight species were selected among the county’s main game and fish species, namely bear, fox, capercaillie pine marten, magpie, hooded crow, trout and grayling. The species were classified based on their results from 19 criteria (harvesting period, harvested quantity by one worker in eight hours, harvesting cost, harvesting knowledge, required instruments, the harvesting process’s complexity, distribution array, biotic threats, abiotic threats, perishability, market potential, market request, the product’s market “popularity”, raw product price, derived product price, derived products portfolio, transport from the harvesting place to the storage center). Grades from 1 to 8 were given to each analyzed species by specialists. As such, the most important species are the trout and, grayling while the species with the smallest percentage were hooded crow and magpie. The species’ harvesting and distribution potential as well as their diversity in the county are high as both the forest and fish areas are very well represented. In this way, the game and fish species with a high interest have the space and environment in which to reproduce and develop.
THE STUDY OF TOLERANCE OF SOME SPECIES AND VARIETIES OF CUCURBITACEAE TO THE ATTACK OF PATHOGENS
ALEXANDRU BUTE, SILVICA AMBĂRUŞ, CREOLA BREZEANU, MARIA CĂLIN, PETRE MARIAN BREZEANU
This is a study of tolerance of some species and varieties of Cucurbitaceae to the attack of pathogens that are based on the scientific results obtained in the field. This paper aims to add studies on disease resistant species, in order to achieve these objective, other intermediate analyzes were necessary which were made using the following indicators: frequency of attack (F%), intensity of attack (I%) and the degree of attack (DA%). The results obtained from this experiment will be used in conventional and organic farming systems in order to decrease the number of diseases treatments and finding and using the most disease and pest resistant varieties of Cucurbitaceae. The trial was performed on the following cultivars: Cucurbita pepo cultivars: V1 – Aredendo T-1, V2 – Local population 2 , V3 – Milet F-1, V4 – Alburiu de Sarzana, V5 – L9 / 2018 Bacau, V6 – Gary Zucchino, V7 – Early Garden, V8 – Winter Squash, V9 – Born Free, V10 – Bush Pink Banana, V11 – Winter Squash Spaghetti, V12 – Local population 4 China, V13 – L 4 Bacau, and V14 - Local population 11. Also, on the Cucurbita moschata V15 – L 5. Luffa cylindrical V16 – L 1. From Lagenaria siceraria the following: V17 – Local population (orange pear), V18 – Local population (green pear), V19 – Local population (small pear) and V20 – Local population green with stripes. From Cucurbita maxima the V21 – Turban and from Momordica charantia V22 – Brâncuși
STUDY ON STAPHYLOCOCCUS AUREUS INFECTION METHICILLIN-RESISTANT (MRSA)
MARIA PRISECARU(1), IONUŢ STOICA(1), RAMONA ONEŢ(2), DANIELA TIŢĂ(3), TATIANA CIUREA(4), FLORIAN PRISECARU(5)
This paper, which proposed a study on methicillin-resistant Staphylococcus aureus (MRSA) infection, is based on analyzes and statistical processing that were performed between July 2019 and March 2020. A retrospective descriptive study was performed in the laboratory of SC medical analysis. HYRON ARS MEDICA S.R.L. from Bacău. The following data were taken from the records made in the Rilab software: name and surname, age, type of analysis requested and also information on pre-existing conditions and treatments recommended by the doctor were taken. The study was performed in patients who were explained the purpose and protocol of the study and who gave their consent to participate. The study group included 1304 patients. Of these, 320 patients were infected with Staphylococcus spp., Aged between 1 month and 83 years, of which 285 patients were under 20 years of age at the time of the study. There has been a significant increase in the percentage of patients in whom Staphylococcus aureus manages to enter, infect and spread in the body, despite the body's own defense mechanisms. An important role in this regard is played by nosocomial infections, which are among the main risk factors in terms of the share of infections.
ECOTOXICITY STUDY ONTHE INFLUENCE OF GLYPHOSATE UPON THE T. AESTIVUM L. DELABRAD 2 CULTIVAR (OECD 208) AND F. CANDIDA (OECD 232)
MARIANA CALARA(1), CARMINA BENCHEA(2), IOAN VIOREL RAŢI(2), DUMITRA RADUCANU(2)
The side effects of pesticides have been constantly studied, and are the subject of books such as the one written by biologist and journalist Rachel Carson who talked about the negative effects of DDT (Dichlor-Diphenyl-Trichloroethane) on the environment in the book "Silent Spring" (Rachel Carson, 1962), a book that would destroy the fame of DDT. Due to undesirable side effects in 1970, DDT was banned in industrialized countries and since 2004 the "Stockholm Convention" has banned its use in agriculture. The human attitude towards pesticides has two aspects: the protection of consumers against pesticide residues in food and the training of those who produce or handle pesticides in the sense of proper use. Whether it is insecticides, fungicides, or herbicides, we must keep in mind that the dose is what makes the poison. The objective of this paper is to test glyphosate in two groups of test organisms, according to the recommendations of the OECD-Organization for Economic Cooperation and Development, respectively on Folsomia candida- (OECD 232) and wheat- (OECD 208). The results obtained after laboratory testing have revealed the harmful effect of this herbicide on wheat at high concentrations (of 5mL/0.25mL water, respectively 7.5mL/0.25mL water), inhibiting the seed germination process as well as the plant growth processesand development and their content in assimilating pigments. Although the mortality rate for adults of F. candida was low, the number of juveniles decreased in direct proportion to the concentration of glyphosate used in the experiment.
STUDY ON THE INCIDENCE OF ANTI-SARS COV2 ANTIBODIES TO HEALTHCARE STAFF
TATIANA CIUREA(1), DANIELA TITA(2), DIANA IOANA TITA(3)
The exact extent to which a person with SARS CoV2 infection can transmit the infection to other is uncertain. The potential for SARS-CoV-2 transmission begins before the onset of symptoms and decreases thereafter. Infected people are more likely to be contagious in the early stages of the disease, when the level of viral RNA in the upper respiratory tract is highest. The duration of viral RNA clearance is variable and may increase with disease severity. In some people, viral RNA can be detected in the respiratory tract a few months after the initial infection. However, detectable viral RNA does not always indicate the presence of the infectious virus and there appears to be a threshold below which the infectivity is unlikely. Prevention and limitation measures are essential in both healthcare and the community. Medical personnel must apply additional precautions to protect themselves and prevent the transmission of the disease during medical care. This paper aims to study the incidence of the IgM and IgG antibodies anti-SARS CoV2 in healthcare professionals. The determination of antibodies was made on request, both to see the immunization of people who went through the disease and to evaluate asymptomatic personnel (they came in direct contact with confirmed people).
DYNAMICS OF SOME ERYTHROCYTIC INDICES IN MEGALOBLASTIC ANEMIA
MARIA PRISECARU(1), IONUŢ STOICA(1), TATIANA CIUREA(2), DANIELA TIŢĂ(3), OANA FLILIP(3), GABRIEL ALIN IOSOB(4), MARIA CĂLIN(5)
Over a period of 2 years, between 2017-2019, a study of erythrocyte parameters was performed in 34 patients with megaloblastic anemia. The complete hemoleukogram accompanied by the peripheral blood smear was used as working methods for the study of erythrocyte parameters. Peripheral blood smear investigations Mean erythrocyte volume (VEM) and mean erythrocyte hemoglobin (HEM) are the most pathologically altered erythrocyte indices. The average erythrocyte hemoglobin concentration (CHEM) is an index within relatively normal limits.
LAND-SNAIL DIVERSITY OF PIATRA-NEAMŢ (NEAMŢ COUNTY, ROMANIA)
STELIAN GRIGORE
This paper presents the list of land-snail to be found within the administrative territory of Piatra-Neamţ (Romania). The material of study, which encompasses more than 1.200 samples, is the result of thirty years of observation and collecting, spanning between 1990 and 2020. We have identified 48 species of land-snail, belonging to 14 families. The area of study includes seven natural protected areas, of which four are of national interest and three are NATURA 2000 sites. The list of land-snail presented (the first on this territory), predominantly comprises continental elements, plus alpine forms, as well as a rare and isolated steppe biocoenosis.
BEHAVIOR OF VEGETABLE PLANT SEEDS AT APHIDS ATACK AND CONTROL OF THESE PESTS
MARIA CALIN(1), CRISTEA TINA OANA(1), SILVICA AMBĂRUŞ(1), CREOLA BREZEANU(1), PETRE MARIAN BREZEANU(1), GABRIEL ALIN IOSOB(1), PETRU SEBASTIAN MUSCALU(1), MARIANA CALARA(1), ALEXANDRU BUTE(1), MARIA PRISECARU(2)
The experiments were carried out at the Vegetable Research - Development Station Bacau in open field crops for seeds: bean, parsley, beetroot, celery, lovage, tomato, pepper, eggplant for control of aphids attack. The degree attack (DA%) of black bean aphid was high on the bean – 24.6 %, followed by seed plants of beetroot 11% , parsley - 7%, lovage - 4.2%, celery – 3.5%. Green peach aphid had the biggest attack on the pepper 14.2%, followed at the eggplant – 7.4%, tomato - 5.6% and bean 0.1%. Green potato aphid had a smaller DA% on: tomato – 2.5%, pepper - 1.4%, eggplant – 0.2%. Potato aphid had a smaller DA% in: tomato – 1.8 %, pepper – 0.9%, eggplant – 0.1%. Cabbage aphid attack only cabbage plant of seeds, DA% being 5.9 %. The efficiency of products studied for the control of black bean aphid attack had very good efficiency. So Teppeki - 0.14 kg/ha, had the best efficiency - 99.5%, followed by Mospilan - 0.15 kg/ha - 99.1%, Konflic - 0.3% - 98.4 and Neemes – 025 % - 94.3%. The efficiency of products studied for the control of green peach aphid attack was very good in all variant being over 90%. All products had very good efficiency in control of green potato aphid, over 93%. In condition of low attack of potato aphid which did not exceeded 1.8% DA, all variants had over 93%. The efficiency of products in control of aphids at plant cabbage for seeds was very good: Konflic - 0.3% - 99.0 %, Neemes – 0.25 % - 94.2 %, Teppeki - 0.14 kg/ha - 99.6%, Mospilan - 0.15 kg/ha - 99.4%
VARIABILITY OF PLEURAL LIQUID CHARACTERISTICS AND THEIR ROLE IN DIAGNOSIS
MARIA PRISECARU(1), IONUŢ STOICA(1), DANIELA TIŢĂ(2), TATIANA CIUREA(3), ANDRA OTELIŢĂ(4), FLORIAN PRISECARU(5), GABRIEL ALIN IOSOB(6)
The paper aims to highlight the value of laboratory investigations in various pleurisy by exploring the pleural fluid on the three levels (biochemical, bacteriological and cytological), correlating it with the clinical and imaging aspect to establish the etiology of pleurisy. The researches were performed in the Clinical Laboratory within the Bacău Pneumoftiziology Hospital between March and May 2020. To study the characteristics of pleural fluids, a number of 56 subjects diagnosed with pleurisy of different etiologies were analyzed. Laboratory tests were performed on pleural fluid obtained by pleural puncture. The patients investigated were grouped by sex, according to background and age groups. The investigation of pleural fluids on the three levels (biochemical, cytological and bacteriological), correlated with clinical manifestations and radiological aspects led to the diagnosis of patients with the following types of pleurisy: tuberculous pleurisy, parapneumonic, neoplastic, eosinophils and pleurisy due to other causes. In all types of pleurisy, male patients have the highest number of cases. All neoplastic pleurisy was recorded in age group III.
DATA CONCERNING THE STRUCTURE OF BENTHIC MACROINVERTEBRATES COMMUNITIES IN THE LOWER BASIN OF THE RIVER MURES (ROMANIA)
ROXANA - ELENA VOICU, CAMELIA URECHE, DOREL URECHE
The study on the structure of benthic macroinvertebrate communities took place in the Lower Mureș defile (May 2011, 18 sampling points). Following the processing of benthos samples, 20485 individuals were identified, belonging to 14 groups of benthic macroinvertebrates.In all sampling points the clean water indicator groups (Amphipoda, Efemeroptera, Plecoptera, Trichoptera) registered a high abundance.
PARASITIC HYMENOPTERA AND THEIR INFLUENCE ON THE NUMBER OF LEPIDOPTERA PESTS FOR BRASSICA OLERACAE L. VAR CAPITATA FROM THE EXPERIMENTAL FIELD OF THE VEGETABLE RESEARCH AND DEVELOPMENT STATION BACĂU
GABRIEL-ALIN IOSOB(1), MARIA CĂLIN(1), TINA OANA CRISTEA(1), SILVICA AMBARUŞ(1), CREOLA BREZEANU(1), PETRE MARIAN BREZEANU(1), MARIA PRISECARU(2), ALEXANDRU BUTE(1), MARIANA CALARA(1), ANDREEA ANTAL TREMURICI(1), CARMINA MIHAELA BENCHEA(1), DANIELA BOURUC(1), DAN-IOAN AVASILOAIEI(1), SEBASTIAN-PETRU MUSCALU(1)
In the experimental fieldon the autumn cabbage, Silviana variety of Vegetable Research and Development Station Bacău, four Lepidoptera species have been identified as major pests (Mamestra brassicae, Discestra trifolii, Pieris brassicae and Plutella xylostella) in 2019. Were collected biologic material represented by clutchs of eggs. In the laboratory, daily observations were made,to see the degree of parasitic attack. The highest density of parasitic Hymenopterawas recorded by T. evanescence at all M. brassicaeeggs.
STUDY ON THE CYTODIAGNOSIS OF DIFFERENT SUBTYPES OF NON – HODGKIN'S LYMPHOMAS
ADRIAN – CONSTANTIN TUDORACHE
Between January 2020 and May 2020 I conducted a study aimed at identifying 4 subtypes of non-Hodgkin lymphoma (hairy cell leukemia, Burkitt lymphoma, T-cell large granular lymphocytic leukemia, T-cell prolymphocytic leukemia). The sample was sampled by 111 people, women and men between the ages of 5 and 81. Interpretations were made by sex, by age group, by type of leukemia, on the chronic or acute side; and in patients with HCL we have detailed the information on laboratory examinations for an overview
AN OVERVIEW ON GENUS ARTEMIA LEACH, 1819 (CRUSTACEA: BRANCHIOPODA, ANOSTRACA) FROM ROMANIA
ALEXIA BERINDEAN(1), KARINA PAULA BATTES(1), BEATRICE KELEMEN(2), MIRELA CÎMPEAN(1)
The genus Artemia, known as the brine shrimp, represents an important inhabitant of hypersaline lakes of Romania. Worldwide, the genus consists of six well-characterized bisexual species and several clonal lineages described with the binomen Artemia parthenogenetica. Despite its conspicuous appearance and numerous records in the literature, dating back to the eighteenth century, no positive identification can be provided for Artemia in Romania: the citations change from Artemia salina, to Artemia sp, Artemiaparthenogenetica, or parthenogenetic strains of Artemia. The present paper represents a review of previous records of the genus in Romania. Further studies are mandatory to clarify all taxonomic issues associated with this genus.
EVALUATION OF SOME METHODS FOR DETERMINING LAMOTRIGINE FROM BIOLOGICAL FLUIDS AND PHARMACEUTICAL PREPARATIONS
TATIANA CIUREA(1), DANIELA TIŢĂ(2), IONUŢ STOICA(3)
Lamotrigine is a broad-spectrum antiepileptic used as monotherapy or as an adjunct to other antiepileptics for the treatment of partial or generalized tonico-clonic seizures. This drug is also used in the treatment of neurological lesions, bipolar disorder, and other psychiatric disorders. The choice of the appropriate method for the routine monitoring of the drug administered to the patient with epilepsy in order to individualize the therapy, the difficulty of establishing the dose, the therapeutic field, the fluctuations of the serum concentration in certain physiological conditions (during pregnancy) imposed this study. The literature presents several methods for determining LMT and metabolites in the biological matrix including: HPLC, gas chromatography with nitrogen-phosphorus detector, mass spectrometry with thermospray chromatography, capillary electrophoresis, radioimmunoassay, immunofluorimetric analysis, UV electrophotometry, methods. In this paper, the focus was on chromatographic and spectrophotometric and electrochemical methods. Electrochemical methods with modified electrodes for LMT dosing are gaining ground lately. The choice of method aims at: simplicity of analysis, precision, accuracy, sensitivity and selectivity of the method, low analysis time, low costs.