The cosmetic industry looks for substances able to preserve and take care of the skin. Biotechnology is one of the way to prepare substances able to satisfy the consumers needs. Lipases and glycosidases constitute enzymes families with strong potentialities for the synthesis of many ingredients. These enzymes work in synthesis as well as hydrolysis and catalyze chemio-, regio- and stereo-selectives reactions. Examples could be given such as the modification of antioxidants like vitamin A and C, the synthesis of natural dyes like indigoids, but also the synthesis of biosurfactants like glucamides.