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DEVELOPMENT OF SPECTROPHOTOMETRICAL ASSAY FOR THE STUDY OF THE INTERACTION OF ANTIOXIDANT STANDARDS WITH 1,1-DIPHENYL-2-PICRYLHYDRAZYL FREE RADICALS


TOUHAMI LANEZ *, HADIA HEMMAMI
University of El Oued, VTRS Laboratory, PO Box 789, 39000, El Oued, Algeria
*Corresponding author: touhami-lanez@univ-eloued.dz

Issue:

SCSCC6, Volume XVI, No. 3

Section:

Volume 16, No. 3 (2015)

Abstract:

The aim of the present study was to develop a spectrophotometric based assay for the determination of binding parameters like binding constant and binding free energy of the free and 1,1-diphenyl-2-picrylhydrazyl (DPPH) bound forms of a set of antioxidant standards (AS). The determination of binding constant and binding free energy is based upon the decrease in absorbance of the electronic absorption spectrum of a 10-4 M acetonitrile solution of DPPH in the presence of a given concentration of a solution of antioxidant standards in the same solvent.

Keywords:

binding constants, free energy, UV-Vis.

Code [ID]:

CSCC6201503V03S01A0004 [0004300]

DOI:

Full paper:

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