Using the statistical analysis and factorial experiments of second order, two mathematical correlations for the folic acid facilitated pertraction using Amberlite LA-2 as carrier have been established. These equations describe the dependences between the solute initial mass flow, respectively the permeability factor, and the main parameters influencing the separation efficiency (pH-values of the feed and stripping phases, carrier concentration). For both cases, the considered variables control the separation process in a 96.1 - 96.8% extent, the carrier concentration exhibiting the most important influence.