Abstract
In this paper, there is tested the influence of pine extract (needles) on the microbiota air from different enclosures: industrial hall, toilet and university yard. The achievement of this study is necessary because the complex microbiota (bacteria, yeasts, molds spores, fungi) in the air have epidemediologically regulated standards (quantitative and qualitative). The microbiological analysis of the air, in the three spaces, is made according to the Koch method (method of air sedimentation). The calculation of NTG (the total number of germs) is achieved using the formula of Omeleanski.
The experiment was conducted during spring time (15-22.03.2009), using the factorial program of type 33. The mathematical modeling of factorial type 33 involves a set of 27 experiments and three additional experiments used as approval samples. (Nistor I.D.).
We considered three levels of variation, as follows: amount of extract (ml);pH of environment of culture medium; time of exposure (minutes).