The experimentations were performed during 2011-2012 in Laboratory of Plant Tissue Culture from Vegetable Research and Development Station Bacau. Regeneration, in all its aspects – callus, shoot and embryo formation and rooting of newly formed plantlets are key points on which relies the entire concept of in vitro tissue culture. Therefore, the main objective of the present study was the assessment callus and shoot induction in white cabbage anthers cultivated on media with different concentration and combination of PGRs. Anther-derived calluses, normally accompanied by elongating small buds, are induced in response to type of auxins added and subsequently to combination with cytokinin, especially 6- benzylaminopurine (BA). The formation of callus structure was the predominant morphogenetic reaction on media with 2,4 D, while NAA improved the embryogenic competence of anthers. The results of the present study also support the idea of major impact involvement of PGRs in the expression of cells totipotence.