The aminoacids that are present in the haploid plants of 10-12 cm length obtained by gynogenesis „in vitro” via the culture of nonfertilized ovaries and ovules have been identified and quantitatively measured in wheat (Triticum aestivum L.), sugarbeet (Beta vulgaris L.), sunflower (Helianthus annuus L.) and potato (Solanum tuberosum L.) by using the chromatographic method upon thin larger and UV-VIS spectrophotometry. Diploid plants of the same length where used for control. They were obtained by germination of the donor plants seeds from where ovaries and ovules for cultivation in vitro were sampled. Thus, it is found that in the haploids obtained in vitro, for all studied species, the values of aminoacids are in general clearly low, as compared to the control diploid plants. The haploid plants chromatograms show also the lack of some aminoacids identified in the control diploid plants. It is interesting to note that in some haploids new aminoacids appear, but they are not identified in the 2n plants (phenylalanine, in the potato haploid), or in almost equal quantitative values, or higher that the 2n donor plant value ( this in the case of methionine in wheat haploid and histidine in sunflower haploid).