CHEMICAL COMPOSITION AND ANTIBACTERIAL PROPERTIES OF ESSENTIAL OILS OF Pimpinella Anisum L. GROWING IN MOROCCO AND YEMEN

A. AL MAOFARI(1), S. EL HAJJAJI(1)*, A. DEBBAB(2), S.ZAYDOUN(1), B. OUAKI(5), R. CHAROF(4), Z. MENNANE(4), A. HAKIKI(3), M. MOSADDAK(3)

1 Université Mohammed V-Agdal, Faculté the Science, Département de Chimie, Laboratoire de Spectroscopie, Modélisation Moléculaire, Matériaux et Environnement, EPCME, Rabat, Morocco
2Heinrich-Heine-Universität Düsseldorf, Institut für Pharmazeutische Biologie und Biotechnologie, Düsseldorf, Germany
3 Université Mohammed V-Agdal, Faculté the Science, Département de Chimie, Laboratoire de substances naturelles et thermolyse éclair, Rabat, Morocco
4 Institut National d'Hygiène, Laboratoire de bactériologie médicale, Rabat, Morocco
5 Ecole Nationale de l'Industrie Minérale, Laboratoire de génie des matériaux, Rabat, Morocco
*Corresponding author: selhajjaji@hotmail.com hajjaji@fsr.ac.ma

Abstract

We report in this study the chemical composition and antibacterial activities of the seed’s essential oils of Pimpinella anisum L. collected from Morocco and Yemen. The hydro-distillation technique was used to extract their essential oils, followed by continuous liquid-liquid fractionation using water and ethyl acetate as solvent system. Obtained essential oils were analyzed by gas chromatography (GC) and gas chromatography-mass spectroscopy (GC-MS). GC and GC/MS results showed that 4-allylanisole was the major compound of Pimpinella anisum L. with percentages of 76.70 and 85.28% of Moroccan and Yemen, respectively, in addition to other minor compounds such as limonene (9.75% for Moroccan species and 5.53% for Yemen species) and fenchone (6.16% for Moroccan species and 4.12% for Yemen species). Furthermore, both essential oils were evaluated for their antibacterial activity against a panel of pathogenic microorganisms. The results showed that both essential oils inhibit most pathogenic bacteria tested.

Keywords

antibacterial essential oils food-borne diseases GC-MS hydro-distillation Pimpinella anisum L.
Full article (PDF)