IN VITRO CULTURE OF UNFERTILIZED OVULES IN BRASSICA OLERACEA L.

PRISECARU MARIA(1), CRISTEA TINA OANA(2), MARIA CĂLIN(3)

1. Faculty of Sciences, Biology Deprtament, University “Vasile Alecsandri” , Marasesti Street no. 157, Bacau, Romania,
e-mail: prisecaru_maria@yahoo.com
2,3 Vegetable Research and Development Station Bacau, calea Barladului no. 220, Romania,
e-mail: tinaoana@yahoo.com;
e-mail: sclbac@legumebac.ro

Abstract

Using the unfertilised ovule culture of 4 genotypes of Brassica oleracea var. capitata (three consanguine lines and one hybrid) haploid plants diploid and mixoploid plants with gynogenetic origin were successfully obtained. A larger number of embryos/callus/ovules and normal plants were obtained. The gynogenesis in vitro through unfertilised ovule culture can be an effective method of production of haploids and dihaploids at white cabbage.

Keywords

Brassica oleracea var. capitata ovule culture embryogenesis haploid plants.