EXTRACTION AND ESTIMATION OF CHLOROPHYLL CONTENT IN CABBAGE SEEDLING LEAVES (BRASSICA OLERACEA L. SILVIANA VARIETY) GROWN IN SOIL CONTAMINATED WITH DIFFERENT CONCENTRATIONS OF CADMIUM AND COPPER

GABRIEL-ALIN IOSOB(1), VALENTIN NEDEFF(2), MARIA PRISECARU(3), TINA OANA CRISTEA(4)

1. Vegetable Research and Development Station Bacău, 220 Calea Bârladului Str., 600388 Bacău and Vasile Alecsandri University of Bacău, Faculty of Engineering, 157 Mărăşeşti Str., 600115 Bacău, România, e-mail: iosob.gabriel@gmail.com;
2. Vasile Alecsandri University of Bacău, Faculty of Engineering, 157 Mărăşeşti Str., 600115 Bacău, România and Gheorghe Ionescu Siseşti Academy of Agricultural and Forest Sciences, 61 Mărăşti Str., 011464 Bucharest, Romania, e-mail: vnedeff@ub.ro;
3. Vasile Alecsandri University of Bacău, Faculty of Biology, 157 Mărăşeşti Str., 600115 Bacău, România, e-mail: prisecaru_maria@yahoo.com;
4. Vegetable Research and Development Station Bacău, 220 Calea Bârladului Str., 600388 Bacău, România, e-mail: tinaoana@yahoo.com

Abstract

Vegetables are essential in human diet and the presence of HM in excess it is a global issue that threaten plant health, wildlife and people [4]. This study was conducted under the protocol OECD 208. And values of Cd and Cu solutions were established in Order no. 756/3 November 1997 with regard to traces of chemical elements in the soil. Chlorophyll pigments evaluation was performed following the protocol of Lichtenthaler (1987) [11]. After data analysis the difference observed between the amount of Chl a and Chl b was very small in all the variants studied. The largest amount of total chlorophyll and the small amount of carotenoids it was determined variant tow (V2 = 100 mg Cu). And coefficient of variation for photosynthetic pigments is not greater than 0.45%.

Keywords

chlorophyll content cabbage Cadmium and Copper toxicity chlorophyll a chlorophyll b carotenoids
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