THE INVESTIGATION OF THE EFFECT OF HIGH CONCENTRATION AND PH ON MID-IR AND THZ SPECTRA OF BSA SOLUTIONS

  • GHEORGHE GRIGORE
    Department of Anatomy, Animal Biology, Animal Fiziology, Biohysics and Neurobiology, University of Bucharest, Biology Faculty, Bucharest, Romania
  • RAZVAN AIRINI
    Department of Anatomy, Animal Biology, Animal Fiziology, Biohysics and Neurobiology, University of Bucharest, Biology Faculty, Bucharest, Romania
  • DAN FLORIN MIHAILESCU
    Department of Anatomy, Animal Biology, Animal Fiziology, Biohysics and Neurobiology, University of Bucharest, Biology Faculty, Bucharest, Romania

Abstract

A natural biological environment involves highly concentrated molecular solutions. In such an environment, the activity of a protein depends on its structure and its conformational changes that appear when the protein interacts with other molecules. The purpose of this study is to elucidate the difference between the dilute and the high concentration solutions of bovine serum albumin (BSA) through THz and FTIR spectroscopy. We then measured the Mid-IR and THz spectra (400-4000 cm-1) for the 96 solutions. Our data show that the absorption value increases in a nonlinear way with the BSA concentration at different pH values, a fact that may be assigned to the high concentration effect.

Cuvinte cheie

high concentration FTIR spectroscopy THz spectroscopy bovine serum albumin