Volume 28, No. 2 (2019)
Articles
BIOLOGICAL AND MORPHOLOGICAL PARTICULARITIES OF THE MAIN SPECIES OF TRICHOGRAMMA, COLLECTED IN THE AGROCENOSIS OF THE REPUBLIC OF MOLDOVA
LIDIA GAVRILITA
As a result of the analysis, the Trichogramma which hatched from the parasitized eggs of the pests collected from nature and eggs exposed to Sitotroga cerealella O., proved to have the following species identified: T. evanescens Westw., T. pintoi Vog , T. semblidis A., T. leucaniae P. & Ch., T. sibiricum Sor., T. dendrolimus Mat, T. aurozum Sug., T. piceum Dj., T. embryophagum Hb., T. sorokina Kost ., T. telengaie Sor. The Trichogramma spp. entomophagus is used in the egg stage to combat various pests: the buch, moth, and albila complex.
IMUNOENZYMATIC RESEARCH IN PANCREATIC DISEASES
MARIA PRISECARU(1), DANIELA TIŢĂ(2), ROIBU LILIA(1), IONUŢ STOICA(1*), GABRIEL ALIN IOSOB(3), MARIA CĂLIN(4), TINA OANA CRISTEA(4), FLORIAN PRISECARU(5)
The studies are aimed at an evaluation of the complex diagnosis of pancreatitis, based on modern methods of investigation that include the use of specific markers that can invalidate or confirm the diagnosis (CA 19-9 and CEA) and the evaluation of lipase and amylase enzymes, in relation to the type of pancreatitis. In our study were included 70 patients admitted to the gastroenterology department, within the Bacau County Emergency Clinical Hospital, between February and May 2018. The distribution of cases according to the sex of the patients showed a slightly higher frequency of the male patients. (56%) and an equal predominance of cases in rural and urban areas regarding the forms of pancreatic pathologies. The results showed a higher number of cases in the age groups between 41 and 60 years. Plasma concentrations of amylase and lipase increase within hours of an episode of acute pancreatitis. Lipase tends to remain high longer than amylase, making it more useful when presentation to the doctor has been delayed by more than 24 hours. Both enzymes can be grown under different conditions, other than pancreatitis. None is useful in monitoring the course of the disease or in predicting the severity in adults.
STAPHYLOCOCCUS INFECTIONS: METHICILLIN HIGH-RESISTANCE AND INCIDENCE BASED ON ENVIRONMENTAL FACTORS AND INDIVIDUAL CHARACTERISTICS OF THE PATIENTS
DIANA-RODICA MIREA(1), MINODORA ELENA ROSALIM(2), RAHELA CARPA(1*)
In 2016, out of 1207 hemocultures from patients at Sibiu county emergency hospital, 7% were infected with Staphylococcus, identifying more cases in the months with higher temperatures. Elderly people were mostly affected, regardless of gender. Due to nosocomial infections, 64% of staphylococci were MRS and they came from the surgical section of the hospital. In the present study, the Staphylococcus hemoculture infection rate was described, aiming the identification of the infections, the assessment of the colonization ratio based on the total number of cases recorded in 2016 at Sibiu county emergency hospital and pointing out of possible correlations between colonization and age, gender, hospital section origin of infections and previous treatment. The main characteristics of the species were identified using Gram staining, microscope examination, catalase test, coagulation test and antibiograms. Staphylococcus species cause a lot of conditions, especially if they are not treated properly.
GLYCATION OF BOVINE SERUM ALBUMIN INVESTIGATED BY TERAHERTZ SPECTROSCOPY
GHEORGHE GRIGORE, RAZVAN AIRINI, DAN FLORIN MIHAILESCU
The process of glycation induces conformational changes that alter serum albumin function. This study aims to investigate the evolution of the glycation process by analyzing the Terahertz (THz) spectra of Bovine Serum Albumin unglycated and glicated with glucose and fructose. After analyzing the results we obtained and comparing the spectra of the three types of solutions we concluded that glycation of BSA by glucose produces more significant changes in the THz spectrum than the glycation by fructose.
THE INVESTIGATION OF THE EFFECT OF HIGH CONCENTRATION AND PH ON MID-IR AND THZ SPECTRA OF BSA SOLUTIONS
GHEORGHE GRIGORE, RAZVAN AIRINI, DAN FLORIN MIHAILESCU
A natural biological environment involves highly concentrated molecular solutions. In such an environment, the activity of a protein depends on its structure and its conformational changes that appear when the protein interacts with other molecules. The purpose of this study is to elucidate the difference between the dilute and the high concentration solutions of bovine serum albumin (BSA) through THz and FTIR spectroscopy. We then measured the Mid-IR and THz spectra (400-4000 cm-1) for the 96 solutions. Our data show that the absorption value increases in a nonlinear way with the BSA concentration at different pH values, a fact that may be assigned to the high concentration effect.
IMUNOENZYMATIC INVESTIGATIONS IN VIRAL INFECTIONS HEPATIC B AND C
MARIA PRISECARU(1), DANIELA TIŢĂ(2), FLORIAN PRISECARU(3), IONUŢ STOICA(1*), GABRIEL ALIN IOSOB(4), TINA OANA CRISTEA(5)
In the present paper, the studies are channeled towards an evaluation of the incidence of infections with hepatic viruses (B and C) by immunoenzymatic methods, using commercial kits. The study was performed on all cases registered in January and February in 2019 (1342 patients). The following markers were used: hepatitis B virus surface antigen (AgHBs) for hepatitis B virus infection and hepatitis C virus antigen antibody (anti-HCV Ac) antibody for hepatitis C virus infection and enzyme transaminases activity was determined (TGO and TGP). The presented results allow us to state that viral hepatitis B and C are still a major public health problem, affecting all age categories of the population (small children and infants, young, adults and elderly). In January and February 2019, the age group most affected by AgHBs was 55-64 years old in men, the age group 25-34 years old being the dominant age group. 50 patients with HCV antibodies were present (4.72%), 25 men (1.86%) and 25 women (1.86%) in January and February. The age group with the highest incidence was 55-64 years in both men (36.36%) and women (36%). For 2019, in the two months of study - January and February - of the 1342 people investigated, 125 were positive for both viral markers, AgHBs and AntiHCV.
STUDY OF TOMATO PESTS IN ORGANIC AGRICULTURE
MARIA CĂLIN(1), TINA OANA CRISTEA(1), SILVICA AMBĂRUŞ(1), CREOLA BREZEANU(1), PETRE MARIAN BREZEANU(1), GABRIEL ALIN IOSOB(2), PETRU SEBASTIAN MUSCALU(1), MARIA PRISECARU(3)
Experiments were carried out at the Bacau Vegetable Research and Development Station Bacau between 2016 and 2019. The main pests in tomato crops were: common trip - Thrips tabaci Lind., corn earworm - Helicoverpa armigera Hbn. and Colorado beetle - Leptinotarsa decemliniata Say. For the control of tomato pests treatments with insecticide products for organic farming were applied: Konflic - 0.3%, ProBalance - 0.3%, Neem oil - 0.3%, Neemex - 0.3%, Bactospeine DF - 0.1%, Laser ™ 240 SC. The best results in the control of onion thrips were Laser ™ 240 SC - 0.04% with 98.5% efficiency. Konflic - 0.3%, Neemex - 0.3% and Bactospeine DF - 0.1% had an efficacy of over 92% in the control of corn earworm larvae, ages 1 - 3. The best effectiveness in control of Colorado potato beetle larvae, ages 1 - 2 had Konflic - 0.3% (95.1%) and Neem Oil - 0.3% (91.4%).
AN INDIRECT METHOD OF RISK ASSESSMENT OF CHILDREN'S CANCER
MARIA PRISECARU(1), LUIZA ANDREI(1), IONUŢ STOICA(1*), MARÍA CĂLIN(2), TINA OANA CRISTEA(2), ALIN GABRIEL IOSOB(3), FLORIAN PRISECARU(4)
Between October 2018 - February 2019, a study was conducted on the degree of risks of developing some diseases, including cancer, on a sample of 53 students, all students being in the 7th grade, aged 13-16 years, the average age being 14 years. The interpretations were made on classes and sexes, following if there are risks of cancer, taking into consideration the following factors: BMI value, skin type, family history, consumption of toxic substances, alcohol and / or tobacco. The results show that there are a significant number of students at high risk for illness.
ECOLOGICAL RESEARCH ON AVIFAUNA OF RESERVOIRS IN THE MIDDLE OF ARGES VALLEY. THE AUTUMNAL SEASON
MARIA DENISA CONETE
In this paper, we present the results of our ecological research on the avifauna of reservoirs from the middle basin of the Argeş River, during the autumnal season. The autumnal season has been relatively rich in species (147 species belonging to 14 orders, 39 families and 91 genera); 57 species are dependent on wetlands. We also perform an analysis of the avifauna according to ecological indices. The Anseriformes were overdominant. During the autumnal season, as well as during the serotinal and the prevernal ones, according to the dominance and the Dzuba index of ecologic significance, 5 species were characteristic: Anas platyrhynchos, Aythya ferina, Fulica atra, Phalacrocorax carbo şi Larus ridibundus. During this season, we can observe evident autumnal agglomerations that are specific to migration, which continues the migration started during the serotinal season. The attractiveness of the five reservoirs for the fauna is different and dynamic, thus registering ample migrations from a reservoir to another. Researched lakes represent, for the migratory aquatic birds, important locations for resting and feeding during their cross over from the nesting areas towards the wintering shelters.
A SYSTEMATIC CONSPECTUS OF THE INVERTEBRATE SPECIES IDENTIFIED IN THE SCREE AND LITHOSOL AREAS FROM THE NORTH -WESTERN SECTOR OF THE LEAOTA MOUNTAINS (SOUTHERN CARPATHIANS), ROMANIA
MAGDALIN LEONARD DOROBĂŢ(1), EUGEN NITZU(2), IONUŢ POPA(2), ANDREI GIURGINCA(2), AUGUSTIN NAE(2), ŞTEFAN BABA(2), CODRUŢA MIHAELA DOBRESCU(1*)
Unlike the Piatra Craiului Massif, spectacular through its developed karstic relief, where multiple caves develop and also, scree and other types of mesovoid shallow substratum (MSS), named also shallow subterranean habitats (SSHs), Leaota Massif has not been studied nearly at all from the micro-fauna perspective, until recent years, in spite of the fact that it is an accessible Massif. Moreover, from the petrology perspective, Leaota Massif presents a much higher diversity compared to the Piatra Craiului Massif; on the surface of these mountains occur sedimentary, metamorphic and also igneous (magmatic) rocks. In fact, we can say that, in Leaota Massif, only the relief developed on sedimentary and metamorphic rocks is defining, as the igneous rocks occurs just as exception, located only as some intrusion. Due to these reasons, during the three years (2014-2016), we have collected the invertebrate fauna from two different type of lithosoil and scree (limestone and crystalline schists), considering that the geological diversity of the substrate leads to a specific diversity of invertebrates. Were identified for the first time in the Leaota Massif 248 species, among these, three new species for Romania's Fauna. This paper is part of a wider project that aims to study the connections between the type of lithologic substrate and the distribution of some zoocenotic components.
VARIATIONS OF SOME MYOCARDIC CITOLIZATION ENZYMES IN ACUTE MYOCARDIC INFARCT
MARIA PRISECARU(1), ADRIANA POPESCU(1), IONUŢ STOICA(1*), DANIELA TIŢĂ(2), GABRIEL ALIN IOSOB(3), MARIA CĂLIN(4), TINA OANA CRISTEA(4)
CK-MB, TGO and LDH enzymes were monitored dynamically in patients diagnosed with IMA. The incidence of coronary heart disease in very young age is low, but it increases progressively with age due to the accumulation of modifiable risk factors (diabetes, high blood pressure, dyslipidemia, obesity, smoking, sedentary lifestyle) and the atherosclerosis process which increases proportionally with aging. In men, the increased incidence of AMI appears in the age group 60-69 years. Male sex is considered a risk factor for cardiovascular disease (70% versus 30% of women). In women, the maximum number of patients is 70-79 years old. Of the 62 patients investigated, two showed no enzymatic changes. The other 60 patients showed increases in these indicators.
STUDY OF THE BACTERIAL LEAF SPOT OF PEPPER – XANTHOMONAS VESICATORIA DOIDGE IN ORGANIC AGRICULTURE
MARIA CĂLIN(1), TINA OANA CRISTEA(1), SILVICA AMBĂRUŞ(1), CREOLA BREZEANU(1), BREZEANU PETRE MARIAN(1), NELLY-LILI FANARU(1), ANA MARIA NECHITA(1), CRINA ANDREEA ANTAL(1), GABRIEL ALIN IOSOB(2), SEBASTIAN PETRU MUSCALU(1), MARIA PRISECARU(3)
In the researches regarding the efficacy of biological products and herbal extracts for the control of Bacterial leaf spot at round pepper, Creola variety was studied at Vegetable Research and Development Station Bacau, in open field conditions. The degree of attack at X. vesicatoria exceeded the economical threshold in 2018. In 2019 the degree attack of Bacterial leaf spot at round pepper did not exceed 1.2%. The best efficacy in the control of Bacterial leaf spot at round pepper in the open field had the variant treated with V5 - The bordeaux mixture - 0.5%, the percentage of efficiency at 7 days after infection with Bacterial leaf spot at round pepper sp. being 84%. It was followed by: V2, Funres – 0,25% with 81.5% efficiency, V1 Condor – 0,25% with 80.0 % efficiency, V3 Blocks – 0,25% with 67.7% efficiency and V4 Nettle maceraate - 0.5% with 35.4% efficiency. Percentage of healthy plants varied depending on the treatment variant, the values being 99.0% in variant 5 treated with The bordeaux mixture 0.5%, 98.8 % in V2 – Funres 0.25%, 98.7% in variant 1 treated with Condor 0.25%, 97.9% in V3 Blocks – 0,25%, and 95.8% in V4 treated with Nettle macerate - 0.5%. In pepper variants the plants attacked by X. vesicatoria during the period of June - the first decade of September resumed their vegetation and fruit set. As a result, GA% decreased progressively. In 2019 the degree attack of Bacterial leaf spot at round pepper was under economical threshold and there were no significant differences between the variants. In the conditions of a very low attack of Bacterial leaf spot at round pepper in 2019, the efficiency of all variants was above 97.7% (V1 Condor – 0,25%, 98.9%: V2 Funres – 0,25%, 99.2%: V3 Blocks – 0,25%, 97.7%:V4 Nettle maceraate - 0.5, 98.1%: V5 The bordeaux mixture 0.5%, 99.2%).
TOPOGRAPHICAL SURVEYS NEEDED FOR THE ASSESSMENT OF OIL AND SALT WATER POLLUTION OF A PASTURE
MĂDĂLINA CRISTINA MARIAN(1), MONICA ANGELA NEBLEA(2), DANIELA GIOSANU(1)
The paper proposes an assessment of polluted land with oil and salt water mixed, having as primarily aim the analysis of a real identification and more precise of the pollution magnitude. The necessity of this study came from the antithesis of a real situation where the oil pollution of an agricultural land used as a pasture seems, at first sight, to have a much larger dimension compared to the results obtained with the use of precision instruments. The rigorous identification of surfaces of wells quadrate, current access roads, underground and above-ground pipelines, surface erosion or deep erosion has made a clear difference between surface degradation through normal operation of crude oil and an excessive, inappropriate activity. Using an equipment and technique with high accuracy of the measurement, the field phase consisted in planimetric and levelling measurements and the collection of soil sampling and stagnant water sampling on the soil surface, from precipitations. The chemical and physical measurements were done in laboratory. In the office work the surveys were processed, the obtained data were systematized and the results were interpreted. The analysis showed that the salinity was the main and the most severely limitative factor in lowering of fertility by slowing the development or even the extinction of microorganims. Although the fodder value of the lawn has been found to be low, however, the grazing in the studied area is practiced. The initial hypothesis concerning the size of the affected surface emphasized an exceeding of the limit of exploitation.
USEFUL BIOMARKERS IN CARDIOPATHY DIAGNOSIS
MARIA PRISECARU(1), DANIELA TIŢĂ(2), OLGA CRAVĂŢ(1), IONUŢ STOICA(1*), GABRIEL ALIN IOSOB(3), FLORIAN PRISECARU(4), MARIA CĂLIN(5), TINA OANA CRISTEA(5)
This paper aims to demonstrate the utility of biochemical markers of myocardial necrosis in diagnosing and managing patients with cardiovascular disease. The studies were performed on 100 patients, between May 2018 - April 2019. Thus, in the peripheral venous blood, the following markers were determined: troponin I, pro BNP, CRP, CK, CK-MB, AST, in patients with heart disease, hospitalized in the sections of cardiology, internal medicine and ATI. All the markers used proved to be useful. Given all this wide range of tests, it remains to be established which of them have a predictive clinical value and to build a multi-marker strategy that involves different pathophysiological aspects, useful in prevention, prognosis, diagnosis and treatment.
DIFFICULTIES, ERRORS AND CORRECTION OF THEM DURING THE COLLECTION OF SAMPLES OR DURING THE MEASURING OF SOME ECOLOGICAL FACTORS (TEMPERATURE AND RELATIVE HUMIDITY) ON THE FIELD
MAGDALIN LEONARD DOROBĂŢ, CODRUŢA MIHAELA DOBRESCU
In this paper are presented various problems that we have encountered in our field research, regarding the monitoring of environmental factors temperature and relative humidity, regarding the collection of faunal material (invertebrates). Sometimes errors have occurred in the case of measurements or for various reasons, for some periods the measurements could not be considered relevant. We tried every time to save the recorded data for the time periods for which they could be considered valid and, of course, we identified the causes of the errors and eliminated them. Sometimes was difficult to understand why for some time intervals the results cannot be considered valid and for other intervals they are valid, we had to differentiate between them. It should be mentioned that permanent monitoring of the main environmental factors temperature and relative humidity, over the months, years, with the taking of the valleys every two hours, both at ground level and at different depths in the mesovoid shallow substratum (MSS), represents a premiere in Romania