ACETIC ACID BACTERIA DETECTION IN WINES BY REAL-TIME PCR

Autori: DAN ZGARDAN(1*), IRINA MITINA(2), VALENTIN MITIN(2), EMILIA BEHTA(1), SILVIA RUBTOV(1), ALINA BOISTEAN(1), RODICA STURZA(1), MARIA MUNTEANU(1)

1. Technical University of Moldova, Faculty of Food Technology, Department of Oenology and Chemistry, 168, Stefan cel Mare Str., Chisinau MD-2004, Republic of Moldova
2. The Institute of Genetics, Physiology and Plant Protection, 20, Padurilor Str., Chisinau MD-2002, Republic of Moldova
*Corresponding author: dan.zgardan@enl.utm.md

Abstract

Acetic acid bacteria (AAB) are considered one of the most common wine spoilage microorganisms. They are still difficult to cultivate on laboratory media, which highlights the importance of alternative methods of detection of these bacteria. The goal of this work was development and testing of a fast and reliable Real Time Polymerase Chain Reaction (RT-PCR)-based method for easy detection of AAB in wine. We designed two primer sets for RT-PCR for detection of AAB and compared the results obtained using these primers with those obtained using a commercial kit. The results obtained using home designed primers showed good correlation with the results, obtained with the commercial screening kit.

Cuvinte cheie

Acetic acid bacteria detection kit DNA primers real-time PCR