Volume 15, No. 2 (2014)
Articles
ANTIBACTERIAL ACTIVITIES OF ETHYL ACETATE EXTRACT FROM Plumeria Alba STEM BARK
MUSTANIR YAHYA(*), HINDIAH M. H., MURNIANA
In current study, antibacterial compounds of ethyl acetate extract from white frangipani (Plumeria alba) stem bark were isolated and their antibacterial activities against Escherichia coli and Staphylococcus aureus were investigated. The plant extract was prepared by maceration of 500 g of dried stem bark P. alba in ethyl acetate. A total of 26 g (5.2 %) of crude plant extract was obtained. The fractionation was carried out by using vacuum liquid chromatography column (VLCC), resulting of five fractions with amount of 0.42 g, 0.36 g, 0.61 g, 0.99 g and 4.15 g for fraction A, B, C, D, and E, respectively. These fractions and crude extract were applied to the Kirby-Bauer method using discs with various concentrations of 0.5, 1.0, 2.0, and 3.0 %, to examine their antibacterial activities against E. coli and S. aureus. The fraction B was found to be the most active, showing average zone of inhibition, at 3.0 % concentration, of 12.4 mm and 15.6 mm to E. coli and S. aureus, respectively. The phytochemical analysis result of fraction B indicated terpenoids compounds. In this experiment, chloramphenicol was used as a positive control.
PREDICTION OF WHITE FLOUR QUALITY OBTAINED BY INDUSTRIAL MILLING OF WHEAT
IULIANA APRODU(1), GEORGETA STOENESCU(2), VIOLETA IONESCU(2), IULIANA BANU(1)*
In order to establish the relations between quality of the wheat and white flour obtained through industrial milling, several parameters related to functional properties of the proteins and starch were analyzed. The parameters defining the proteins functionality are wet gluten, Gluten index, deformation energy of dough and minimum torque C2 and allowed establishing significant correlations between wheat and flour. Concerning the starch baking performance, the parameters that allowed establishing significant correlations between wheat and flour are falling number value, amylase activity, starch gelatinization and cooking stability range. Analyzing the trend of variation of the quality parameters given by Mixolab and Alveograph tests for wheat and flour, one can see that it is possible to predict the flour quality based on wheat quality.
CATECHOLAMINES AND RELATED COMPOUNDS SEPARATION ON A MIXED MODE COLUMN </br> SEPARATION DE CATECHOLAMINES ET MOLECULES APPARENTEES SUR UNE COLONNE EN MODE MIXTE
RALUCA-IOANA TAMPU(1*), OANA-IRINA PATRICIU(1), CLAIRE ELFAKIR(2)
The present paper presents the evaluation of a mixed mode column (reversed phase and cation exchange) performance for the separation of 12 catecholamines, indolamines and their precursors and metabolites. The influence of different parameters, like organic modifier nature and percentage, salts nature and percentage, on the compounds separation was investigated. Good separation of the 12 selected compounds was obtained in the isocratic mode using a mobile phase composed of 90 % methanol and 10% ammonium acetate 20 mM, however the analysis time was excessively long (80 minutes). Thus an elution gradient was optimized and it reduced the analysis time to less than half (35 minutes).
PREPARATION AND PHYSICOCHEMICAL CHARACTERIZATION OF MODIFIED (ACETYLATED) GADUNG (DIOSCOREA HISPIDA DENNST) FLOURS
ANDRI C. KUMORO(*), RIZKA AMALIA, DIAH S. RETNOWATI, CATARINA S. BUDIYATI, RATNAWATI RATNAWATI
Acetylation is one of methods to alter the physicochemical properties of starch. This work aimed to investigate the effect of reaction time, glacial acetic acid/gadung flour (GAA/GF) mass ratio and pH on gadung (Dioscorea hispida dennst) flour acetylation at ambient temperature. The acetylation was carried out by reacting gadung flour slurry with GAA under alkaline condition. The results show that degree of substitution and swelling power of the acetylated flours increased with reaction time, while the solubility was not affected by reaction time after 10 minutes acetylation. The GAA/GF mass ratio inversely affected the solubility of acetylated flour, but did not affect the swelling power and degree of substitution. Acetylation changed the structure, morphology and crystallinity of gadung flour starch granules. The swelling power and solubility of all acetylated flours obtained in this work were higher than the native one.
NEW HYDROGENOMOLYBDATO SnPh2 AND SnPh3 RESIDUE CONTAINING ADDUCT AND DERIVATIVE: SYNTHESIS AND INFRARED STUDY
SERIGNE FALLOU POUYE(1*), IBRAHIMA CISSE(1), LIBASSE DIOP(1), LAMINE A.DIOP(2)
Three new organostannic complex, adduct and derivative have been synthesized and studied by infrared. Dimeric structures involving the cations were suggested on the basis of infrared data. The molybdate anion behaves as a non coordinating ligand-only involved in hydrogen bonds-, mono- or bicoordinating and always involved in hydrogen bonds. The environment of the tin centres is trigonal bipyramidal or octahedral.
BIOLOGICAL TREATMENT OF STABILIZED LANDFILL LEACHATE PRODUCED BY THE PUBLIC DISCHARGE OF THE CITY MOHAMMEDIA (MOROCCO) </br> TRAITEMENT BIOLOGIQUE DES LIXIVIATES STABILISES PRODUITS PAR LA DECHARGE PUBLIQUE DE LA VILLE DE MOHAMMEDIA (MAROC)
ABDELAZIZ MADINZI(1), MOULAY ABDELAZIZ ABOULHASSANE(2), MOHAMMED ASSOU(1*), HAMID FAKIDI(1), MOULAY ABDELLAH BAHLAOUI(1), SALAH SOUABI(1), MOHAMED HAFIDI(3)
In this work, we worked on the removal of pollution discharges stabilized leachate produced by the landfill of the city of Mohammedia using the process of the biodegradation of organic pollutants in air continuously or discontinuously. In order to predict the efficiency of a biological treatment for this type of effluent while minimizing the cost of ventilation. Landfill leachates produced by fermentation of waste are considered as a dangerous source of pollution to the environment. These waters are loaded with highly toxic metal and organic pollutants. They are difficult to treat and constitute a pollutant element for their quantitative and qualitative aspect. Discharges used in this study are characterized by a charge rich of high organic pollution more or less biodegradable, and whose NTK often greatly exceeds 1 g.L-1. The physico-chemical characteristics of leachate showed that the organic mater is readily biodegradable since COD/BOD5 ratio varies between 4.8 and 7.4. The results of this study showed that the continuous ventilation discharge leachate has a removal efficiency of 48 % COD neighbor which remains close to that obtained with the discontinuous aeration (52 %). This is accompanied by a variation of pH and the quantity of sludge biodegradation. No differences were observed between the aeration continuous and discontinuous.
SYNTHESIS, INFRARED AND NMR STUDIES OF SOME SULFATO DIORGANOSTANNIC DERIVATIVES
DAOUDA NDOYE, LIBASSE DIOP(*)
EthylendiammoniumSO4•SnBu2Cl2•1/4ethylendiammoniumCl2 (A), 2ethylendiammoniumSO4•SnBu2Cl2 (B) and Cy2NH2SO4H•Cy2NH2SnBu2Cl3 (C) complexes have been obtained on allowing ethylendiammoniumSO4 and Cy2NH2HSO4 to react respectively with SnBu2Cl2 in specific ratios. The molecular structures of these compounds have been determined on the basis of infrared and NMR data. The suggested structures are dimeric and tetrameric, the tin atom being hexacoordinated, the sulphate anions behaving as a monochelating ligand or non-coordinating and only involved in hydrogen bonds. The hydrogenosulfate is a non-sigma coordinating anion but only involved in hydrogen bonds of acetic acid type or NH…O bonds. The presence of dimeric [(SnBu2Cl3)2]2- in one of the structures is noteworthy. The key role of the cations involved in hydrogen bonds is outlined.
NEW HYDROGENOXALATO ADDUCTS AND MALONATO COMPLEX: SYNTHESIS AND SPECTROSCOPIC STUDIES
MOUHAMADOU BIRAME DIOP, LIBASSE DIOP(*)
Two new hydrogenoxalato and one malonato adduct and complex have been synthesized and studied by infrared and NMR spectroscopies. The suggested structures are discrete, the hydrogenoxalate behaving as a monodentate ligand or only involved in hydrogen bonding, the environment around the tin (IV) centre being tetrahedral or trigonal bipyramidal. The malonate anion is a monodentate ligand. In all the suggested structures, when extra hydrogen bonds are considered, supramolecular architectures are obtained.
SnPh3OH.Ph3AsO·H2O: SYNTHESIS AND SPECTROSCOPIC STUDIES
YAYA SOW(*), ARIMAN YAVI KOCHIKPA OKIO, LIBASSE DIOP
On allowing SnPh3OH to react with Ph3AsO, the adduct SnPh3OH.Ph3AsO·H2O is obtained, its infrared and Mössbauer studies carried out. The coordination of Ph3AsO to the tin (IV) centre leads to an infinite chain structure containing a trans bipyramidal trigonal SnC3O2 arrangement with OH and Ph3AsO being in apical positions. The water molecule bridges through hydrogen bonds the Lewis base and SnPh3OH.
Ph3CCOOSnPh3.Ph3PO AND Ph3CCOOSnPh3.Ph3AsO: SYNTHESIS AND INFRARED STUDY
ABDOU MBAYE, ALEXIS BIENVENU BELEKE, CHEIKH ABDOUL KHADIR DIOP(*), LIBASSE DIOP
The mixture of ethanolic solutions of Ph3CCOOSnPh3 and Ph3PO or Ph3AsO gives Ph3CCOOSnPh3.Ph3PO and Ph3CCOOSnPh3.Ph3AsO adducts which have been characterized by infrared spectroscopy. A discrete structure is suggested for both, the environment around the tin centre being trigonal bipyramidal, the triphenylacetate anion behaving as a mondentate ligand