Volume 25, No. 1 (2016)
Articles
THE BIOLOGICAL SUBSTANTIATION OF APPLICATION OF ANTIMICROBIAL PROPERTIES OF RHEUM RHAPONTICUM IN PLANT PROTECTION
ALLA GLADCAIA, LEONID VOLOSCIUC
The most of plant secondary metabolites protect plants against pathogens. Polyphenols and their derivatives play an important role in protection of plants against the fungal diseases. We studied the Rheum rhaponticum L. (Polygonaceae) alcoholic extract in order to reveal the anthraquinone derivatives which induce resistance to plant phytopathogens and act as an antimicrobial agent with fungicidal activity. An alcoholic extract of Rheum rhaponticum L. was prepared and analyzed using chromatography.
ANTHOCYANIN CONTENT AND COMPOSITION OF FRESH AND DRY POMACE FROM VITIS VINIFERA L. WINE CULTIVARS
RĂZVAN V. FILIMON(1), ROXANA FILIMON(1), LILIANA ROTARU(2), MARIUS NICULAUA(3)
By-products resulting from grape processing currently represent an environmental issue, their recovery and reintroduction in the food industry is one of the main goals of sustainable agriculture. Anthocyanin content and composition of grapes and their fresh and dry pomace (after processing) of three Vitis vinifera L. wine cultivars growing in Iasi vineyard, NE of Romania, was evaluated in order to identify new sustainable sources of vegetal pigments. HPLC-DAD/ESI-MS anthocyanin profile was unique and included five monoglucosyl forms and four acylated forms of anthocyanins, with malvidin-3-glucoside as main representative. Other acylated forms were also present in the profile as traces. Grape processing and pomace drying resulted in a decrease of total pigment content, mainly for esterified anthocyanins (acetylated and coumaroylated). After grape pressing, fresh pomace of Merlot grapes retained the highest percentage of anthocyanins comparing with raw material (45%). Important quantities of anthocyanins justifies the extraction of these phenolic compounds from the considered material and demonstrate that grape pomace, both in fresh and dry state, is a valuable and accessible source of pigment.
RESEARCH ON THE INTRODUCTION OF THE SPECIESARONIA MELANOCARPA, WITH A HIGH NATURAL VALUE, FROM THE SPONTANEOUS FLORA INTO ORGANIC FARMS
IOAN VIOREL RAŢI, DUMITRA RĂDUCANU, NICOLETA BĂDĂLUŢĂ
Fruit shrubs are species with moderate environmental factors requirements; therefore behave very well in Romania. These shrubs are growing easily and yield fruits quickly, and the obtained fruits have high nutritious, economical and therapeutic value. The measurements applied in this study enable better understanding of species and varieties in terms of organic farming and productivity testing that support the promotion of these species (Aronia melanocarpa) as suitable for orchards. The species is well suited for organic breeding, the success of dry cuttings being 80.5%, whereas the percentage of seed germination is 90-100% successful.
CHANGES IN YIELD AND QUALITY CARACTERISTICS OF VITIS VINIFERA L. CV. MUSCAT DE HAMBURG UNDER THE INFLUENCE OF GIBBERELIC ACID (GA3)
ROXANA COŢOVANU FILIMON(1), RĂZVAN V. FILIMON(1), LILIANA ROTARU(2)
The aim of the work was to determine the optimal dose of gibberelic acid (GA3) to be applied to achieve a higher yield and a harmonization of quality features at the grapevine cultivar for table grapes Muscat de Hamburg grown in Iasi vineyard, NE of Romania, in two years of harvest. Treatments were performed by spraying the inflorescences with 25 ppm, 50 ppm and 100 ppm GA3. The optimal dose of GA3 applied was 25 ppm, resulting in a higher yield and quality of grapes. Lower GA3 doses resulted in an equilibrated sugars/acid ratio, a higher accumulation of anthocyanins and phenolic compounds in berries, improving the commercial aspect of grapes by increasing color intensity and uniformity. The treatment led to an increase in production of over 18% and to a percentage of marketed production up to 74%. This variety develope a tolerance at high concentrations of GA3. The foliar photosynthetic activity was more intense causing an increasing intracellularly CO2 concentration.
DETERMINATION OF NUTRITIONAL ACTIONS OF ASFAC BCO-4 STIMULATOR IN FRUIT TREES CULTURE IN ECOLOGICAL SYSTEM
SIMONA ANDREI(1), IOAN-VIOREL RAŢI(2), DUMITRA RĂDUCANU(2), NICOLETA BĂDĂLUŢĂ(2)
Increased fruit consumption urges manufacturers to develop effective techniques to improve production. Moreover, a major market demand is ecological fruits, leading to the emergence of a large number of organic fertilizers. The aim of this paper is to demonstrate the superior quality of the ASFAC BCO-4 organic fertilizer, regarding biometric measurements of fruits, bio-indicators and phytochemical indicators. Thus, the action of this fertilizer was highlighted for three apple varieties: Jonathan, Idared and Golden Delicious. The treatment with the ASFAC BCO-4 fertilizer was applied at two important stages of the growing season: beginning of flowering phenophase and petal shedding phenophase. A first observation was a major increase of the yield for the fruits obtained after the treatment. Also, important growth increase were observed for bio-parameters. Another important result observed for all the studied varieties was the increase of chlorophyll a and b content, leading to an intensification of the photosynthesis process.
ECOLOGICAL FACTORS IN THE COLLUVIAL MESOVOID SHALLOW SUBSTRATUM, LIMESTONE SCREE, CĂPĂŢÂNA MOUNTAIN (LEAOTA MASSIF), WINTER 2014-2015
MAGDALIN LEONARD DOROBĂŢ(1), AUGUSTIN NAE(2), IONUŢ POPA(2), CODRUŢA MIHAELA DOBRESCU(1)
This paper present and discussed the results of the monitoring of two ecological factors, the temperature and the relative humidity monitorised during winter 2014 -2015, in an ecological station in the in the north-western area of Leaota Mountains. In this station, three polls were located in areas with limestone forming mesovoid shallow substratum (MSS), also called shallow subterranean habitats(SSHs). The first survey has 1m deepth, the second has 0.75m depth and the third was drilled up to 0.5m depth. In each survey was placed a datalogger above a Barber trap, wich recorded continuously for the winter 2014-2015 relative humidity and temperature. The values of this abiotic parameters were collected on every twelve hours. The continuous data collection on ecological parameters mentioned before is a first for Romania.
OBSERVATIONS ON THE INFLUENCE OF SALICYLIC ACID ON RESISTANCE OF RAPHANUS SATIVUS L. VARIETIES TO SALINITY
NICOLETA BĂDĂLUŢĂ, DUMITRA RĂDUCANU, IOAN VIOREL RAŢI
Salicylic acid is a phytohormone which stimulates cell proliferation in small amounts and in large quantities it induces cell death phenomena. Salicylic acid is involved in mediating systemic resistance in plants against phytopathogenic agents, viral, bacterial or fungal; it is an important "signal" molecule involved in developmental processes in plants, in plant stress reactions. As growth and development of plants are strongly affected by environmental factors (heavy metals, salinity, water scarcity, cold, UV radiation, etc.), in this study we aimed to test the influence of the treatment with salicylic acid (0,001mM) on the Raphanus sativus L. varieties at different concentrations of sodium chloride (0.65%, 1%, 1.5%, 2% ). The samples of seeds were divided into three groups: control, the group wetted with solutions of sodium chloride and the group treated with solution of salicylic acid and wetted with various concentrations of sodium chloride. After the germination of seeds the following physiological measurements were carried out: germination rate, biometric measurements (root length, hypocotyl length, width cotyledon, fresh medium weight), dry substance and water content, assimilating pigments content. The varieties of radish, Cherry belle (red radish) and Icicle (white radish), showed small to medium resistance to the action of sodium chloride; treatment with salicylic acid did not stimulate seed germination, plantlets growth or assimilating pigment biosynthesis, exhibiting a mild inhibitor of physiological processes in conditions of salinity.
PHYSIOLOGICAL AND ANTIOXIDANT RESPONSES OF ASPLENIUM SCOLOPENDRIUM TO ELEVATED AMOUNTS OF LEAD IN SOIL
OANA ALEXANDRA DRĂGHICEANU, LILIANA CRISTINA SOARE, MONICA POPESCU
The heavy metal lead is a persistent environmental pollutant. Our studies were carried out to evaluate physiological response and defensive potential of Asplenium scolopendrium exposed to different concentrations of lead added in soil as lead acetate. Plants were exposed to 0, 250, 500, 1000 and 1500 mg Kg-1 Pb+2 for 3 months. Plants possessed efficient enzymatic antioxidant defense mechanism that played important role in limiting stress. The physiological response of Asplenium scolopendrium was investigated by photosynthesis rate and assimilatory pigments (chlorophyll and carotenoids), while the antioxidant defense was evaluated by catalase activity. After 3 months of influencing, the photosynthesis rate is significantly inhibited by high concentration of lead (1500 mg Kg-1 Pb+2). Lower concentrations of lead not produced significant changes in the intensity of photosynthesis. Chlorophyll and carotenoid contents were not affected significantly by Pb treatment. The activity of catalase noted an increase with increasing amount of lead in the soil (an increase of 30.86% in V4 plants, in presence of 1500 mg Kg-1 Pb+2, compared to the control plants).
RESEARCH REGARDING THE INFLUENCE OF ASFAC BC04 BIOSTIMULATOR ON CHERRY – TREE NANA VARIETY
DUMITRA RĂDUCANU, IOAN VIOREL RAŢI, NICOLETA BĂDĂLUŢĂ
Nana variety was obtained in Romania from a Crişana clone at the Research and Development Station for Fruit Tree Growing Băneasa. It is a variety with a wide ecological plasticity, cultivated throughout the country. The fruit is red burgundy, medium size, with spherical shape. Harvest at maturity starts at the end of June and lasts until the end of July. The study presents the comparative analysis of Nana cherry-tree variety when applied the biostimulator ASFAC BC04 and in its absence. The aim of the study was to highlight the morphological, biochemical and productivity differences of Nana cherry-tree variety with and without treatment. We determined some morphological parameters of fruits and stems, fruits and stems biomass, some biochemical parameters (dry matter and water content, sugar content, acidity), assimilation of leaf pigment content. After the treatment with the biostimulator ASFAC BC04 there was an increase in some analysed parameters: fruit weight (3.31%); sugar content (1.11° Brix), chlorophyll content (0.057 mg/g).
ECOLOGICAL CHARACTERISATION OF THE CORMOPHYTE FLORA FROM VALEA RECE BROOKBASIN (HARGHITA COUNTY)
MILIAN GURĂU(1), PETRONELA BRAN(2)
In an area with pastures and forests at the middle mountain level where the limous rocky relief enabled extending the plant list with some rare species in Romania, the spectrum of bioforms is similar to the one encountered countrywide. The interpretations for ecological factors were made for the entire plant list, which explains why it provides general information corresponding to the status of area of protective and economic interest. The list of plants does not have only the value of a species inventory, but also one of monitoring that needs to be taken into consideration on account of the fact that the area is within a Natura 2000 protected site.
MORPHOMETRIC VARIABILITY OF SEA BUCKTHORN SEEDS FROM WILD POPULATIONS AND CULTURE
NICOLETA BĂDĂLUTA, GOGU GHIORGHIŢĂ, IOAN VIOREL RAŢI, DANIELA NICUŢĂ
Hippophaë genre shows an amazing diversity. Morphological differences in the gender make particular reference to the shape, color of fruits and seeds, color and leaves disposition on shoots, etc. The paper presents some aspects of seed biometry of six varieties of sea buckthorn and four wild biotypes grown in the experimental field FRUCTEX Bacau, compared with 31 spontaneous biotypes collected from Bacau, Neamt, Vaslui, Vrancea. We analyzed the following morphological parameters: length, width and seed biomass, after which subsequently recorded values were statistically processed. Length seeds character showed a low variability and the width and biomass seeds showed a medium to high variability.
INVESTIGATIONS ON NEW DEVELOPED SAVORY VARIETY “DARIA”
CREOLA BREZEANU(1), PETRE MARIAN BREZEANU(2), SILVICA AMBARUS(2), TEODOR ROBU(3), AURELIA DOBRESCU(4), MARIA CALIN(2), TINA OANA CRISTEA(2)
Spicy and aromatic plants constitute an important natural resource of our country, their importance is given to social and economic value and the role of food and therapeutic. Value food and therapeutic herbs and seasoning is determined by great complexity and diversity in biochemical constituents. Worldwide, spicy and aromatic plants enjoys growing attention and are the subject of numerous research physicochemical and pharmacological. The study presents results of investigations performed by the variety of savory Daria, Bacau VRDS created and patented under the number 00124 / 28.03.2008. The variety is distinguished by normal variation. Our investigations have involved a comparative analysis of biochemically biological material grown in conventional and organic sitem. Volatile oil content was higher in organic culture.
STUDY OF SPECIES, VARIETIES AND HYBRIDS SUITABILITY FOR ORGANIC FARMING
SILVICA AMBĂRUŞ, MARIA CĂLIN, PETRE MARIAN BREZEANU, CREOLA BREZEANU, TINA OANA CRISTEA, LUCIAN STOIAN
This paper presents a study of species, varieties and hybrids suitability for cultivation in organic agriculture. The study of assortment were performed using methods and practices of organic farming - culture in tunnels and culture in open field - to the following species and cultivars: tomatoes, eggplants, pepper and cucumber. All vegetables have a good ability for cultivation in organic agriculture.
RESEARCH ON THE VARIABILITY OF SOME MORPHOLOGICAL CHARACTERS AND THE BIOMASS OF SEA BUCKTHORN FALSE FRUITS HARVESTED IN ROMANIA
NICOLETA BĂDALUŢĂ, GOGU GHIORGHIŢĂ, IOAN VIOREL RAŢI, DANIELA NICUŢĂ
In Romania, the species Hippophaë rhamnoides L. confirms the genetic variability of the genus Hippophaë and it highlights biotypes with fruits of various sizes, colors spreaded throughout the country. This study aimed some aspects of sea buckthorn cultivars and biotypes phenotypic variability collected from some districts of Moldova region. We targeted that each individual plant undergoing morphological observations to be different in terms of size, shape and color of the false fruit, in order to highlight significant differences between morphology and the area where the plant material was harvested. We analyzed the length, diameter and biomass of false fruit of Hippophaë rhamnoides L. and the values obtained were statistically interpreted. The average length of false fruits oscillated between 5,69 mm (Silvia cultivar) and 11,67 mm (Delta 60 M biotype), the average diameter of false fruits between 4,85 (Letea-2 biotype) and 9,64 mm (Ovidiu cultivar) and average biomass of 100 false fruits registered values between 14,50 g (Gheraiesti-3 biotype) and 44,50 g (Victoria cultivar).
SOME ASPECTS REGARDING THE PRESERVATION STATUS OF THE 4060 HABITAT – ALPINE AND BOREAL SHRUBS FROM THE CĂLIMANI NATIONAL PARK
DANIEL IOAN MAFTEI(1), DIANA ELENA MAFTEI(2)
The 4060 habitat – Alpine and boreal shrubs - is an important habitat in Europe due to its floristic diversity, as it represents a food, nesting and shelter source for the biodiversity in the area. Being submitted to many antropic and natural threats, this habitat may suffer a negative evolution concerning the preservation status. This present paper aims to describe several issues on the preservation state of this type of habitat, as well as the threats and regulations in order to diminish the impact. The purpose is to maintain the biodiversity in the area unaltered in the near future.
RESEARCH ON HIGH-POTENTIAL BIOTYPES OF SEA-BUCKTHORN (HIPPÖPHAE RHAMNOIDES L.) FOR PROMOTING THEM IN IMPROVEMENT WORKS AND ECOLOGICAL PLANTATIONS
IOAN VIOREL RAŢI, NICOLETA BĂDĂLUŢĂ, DUMITRA RĂDUCANU
Sea-buckthorn, besides being considered the “Romanian ginseng” or “divine oil” due to its therapeutic properties, is also a species that can bring substantial income to those who want to develop a business in this area. This study is intended to become a tool in this respect and also a way to promote this miraculous plant, a plantation model for people interested in ecological farming who want to access European funds alotted to this type of plantations.
USE OF IN VITRO CULTURES TO OBTAIN PLANT MATERIAL AT THE SPECIES VERONICA OFFICINALIS L., NEEDED TO MAKE BIOPRODUCTS
DANIELA NICUŢĂ
Veronica officinalis L., native to Europe and Western Asia, commonly known as speedwell, gypsyweed or Pauls’ betony etc., is used as a medicinal plant because of its content of tannins, bitter substances, volatile oil, saponins, flavones and glycosides. Because of these bioactive compounds is recommended for its actions and effects both internally – stomachic, cholagogue, digestive tonic, gentle expectorant, anti-catarrhal, diuretic, astringent, hemostatic, cleansing, anti-inflammatory –, and externally – healing skin wounds and scars, astringent, hemostatic. Among the many advantages provided by the in vitro culture technology at plants, there are: the possibility to preserve the valuable qualities of a genotype by cloning it, as well as the induction of genetic variability in order to obtain genotypes (somaclones) that are more valuable in terms of the content of bioactive compounds. To initiate in vitro cultures of Veronica officinalis L. there were used young shoots harvested from a population at wild flora. As a basic nutrient medium there was used Murashige-Skoog (1962), enriched with several combinations and amounts of growth regulators. Our observations led to the following conclusions: on most nutrient media supplemented with growth regulators, the node and apex explants had a good reaction, generating new shoots and roots. The aspect and number of shoots and the rizogenetic intensity varied depending on the type and concentration of the growth regulators present in the nutrient medium. A proper morphogenetic response was noticed for the explants inoculated on the medium variants of MS enriched with 1mg/l BAP (benzylaminopurine) + 0.1 mg/l IAA (indole-3-acetic acid), 1mg/l BAP (benzylaminopurine) + 0.5 mg/l ANA (naphtyl-acetic acid) and 1mg/l Kin (kinetine) + 0.5 mg/l ANA (naphtyl-acetic acid). The calusogenetic reaction at the inoculated explants was highlighted sporadically, with low yields on some hormonal formulas. The acclimatisation of the vitroplants to the ex vitro environment encountered no problems, and took place in a hydroponic system.
CONTRIBUTIONS FOR HOMOLOGATION OF SOME SEA-BUCKTHORN VARIETIES (HIPPÖPHAE RHAMNOIDES L.) FOR EXPLOITATION IN ORGANIC FARMS
IOAN VIOREL RAŢI, NICOLETA BĂDĂLUŢĂ, DUMITRA RĂDUCANU
Sea-buckthorn is a dioecious species, therefore the planting scheme in a culture is recommended to have one male individual followed by seven female individuals to provide optimal pollination and to get as rich a harvest as possible. The study proposes observations and measurements on the varieties of sea-buckthorn homologated at Fructex Bacău (Diana, Silvia, Tiberiu, Auraş, Ovidiu, Serpenta and Victoria) and of a hybrid male sea-buckthorn entitled Star proposed for patenting, grown organically, as well as their description according to the UPOV sheet.
SUGGESTIONS FOR THE ANALYSIS OF SOME SPONTANEOUS PLANTS GROWING ON ROMANIAN TERRITORY, CONTAINING NATURAL COMPOUNDS OF THERAPEUTIC INTEREST
MILIAN GURĂU
Over the past decades, there has been a fashionable interest in phytotherapy. Hence, the chances of solving health problems have increased and a great diversity of species and substances have been made known. The current paper presents a comparative analysis of the medicinal flora of the 80s with the one of 2015, resulting in discovering no less than 150 species which are not present in „Farmacia naturii” written in 1976-1977. The Medicinal Flora of India comprises more than 2000 species, of which 210 are spread in both Europe and Asia. These species also grow in Romania (over 25% according to the last updating), but 40 are not known yet to Romanians, who are familiar only with related species with less currency in the area such as: Ceratophyllum demersum, Descurainia sophia, Pyrola rotundifolia, Pulicaria dysenterica, Rumex maritimum, Stellaria aquatica, Polygonatum verticillatum, Sisymbrium loeseli(the seeds contain erucic acid), Scutellaria galericulata, Viola reichenbackiana, Viola biflora, etc. The dictionary of flavonoids includes nearly 20,000 worldwide substances, whereas our paper recorded almost 300 species of flowering plants and 13 species of bryophytes present on our territory. By comparing the lists of Farmacia naturii 1977-1978, we found 135 new species, which have been only sporadically studied over the last 2 decades. We do not know the quantitative values although some species have a high number of substances, so we bring to attention some rare protected species, from which such substances should be collected only if the rare plants of therapeutic interest are cultivated. Unless we do this, we risk destroying in a couple of decades the diversity of the medicinal species.
OBSERVATIONS ON THE MORPHOGENETIC REACTION OF THYMUS VULGARIS EXPLANTS CULTIVATED IN VITRO
DANIELA NICUŢĂ, NICOLETA BĂDĂLUŢĂ
Thymus vulgaris L. (thyme), belonging to the family Lamiaceae (Labiate), is a species originating in the Mediterranean region, having been grown as medicinal and spice plant since antiquity. The bioactive compounds identified in thyme plants are represented by flavonoids, thymol, carvacrol, eugenol, phenols, luteolin, tymol, terpenoids that cause such effects as antispasmodic, bactericide, antiseptic, antioxidant, having anthelmintic properties and having been lately recommended as substitute for cancer prevention agents. Due to its economic importance, the purpose of our research was to highlight the in vitro reaction of this species by observations on the morphogenetic response of a series of explants on various nutritive variants. In view of initiating the in vitro cultures, the biological material used in our research consisted of Thymus vulgaris seeds, disinfested with chloramine-T 5% solution, for 20 minutes. After the removal of disinfesting solutions (by rinsing in distilled water), the seeds were inoculated on Murashige-Skoog (MS) without hormones. The plants obtained from the seeds were used as a source of explants (phytoinocul), represented by shoot apexes and nodes, were inoculated into nutrient media supplemented with the phytohormones from the category of auxins and cytokinins in different combinations and concentrations. Our observations led to the following conclusions: the best reaction highlighted was caulogenesis, followed by rhizogenesis and, to a small percentage by callusogenesis. The media BA1 - 1mg/l BAP (benzylaminopurine) + 0.5 mg/l IAA (indole-3-acetic acid), and BB2 - 1mg/l BAP (benzylaminopurine) + 0.1 mg/l IBA (indole-3-butyric acid) proved to be the most favourable to the regeneration of shoots, followed by the versions BA2 - 1mg/l BAP (benzylaminopurine) + 0.1 mg/l IAA (indole-3-acetic acid) and BB1- 1mg/l BAP (benzylaminopurine) + 0.5 mg/l IBA (indole-3-butyric acid), where the shooting phenomenon was intense, but the shoots were frail, with smaller leaves and thinner stems. Thyme callus formation was highlighted in the medium version NAA1, the callus showing low proliferation and organogenetic capacity.
RHODIOLA ROSEA L. 'S PRESERVATION STATUS IN THE CĂLIMANI NATIONAL PARK
DIANA ELENA MAFTEI
Rhodiola rosea L. is a a major phytopharmaceutically important species that grows spontaneously in our country. This paper aims to analyze the Rhodiola rosea’s preservation status within the Călimani National Park, the types of habitats in which this species was found, the relationship with the natural environment, the threats and the management proposals for a sustainable development.
PRELIMINARY DATA ON THE PRESERVATION STATUS OF RUSCUS ACULEATUS L. SPECIES WITHIN THE TĂŞAD NATURA 2000 SITE
DIANA - ELENA MAFTEI(1), DANIEL – IOAN MAFTEI(2)
The preliminary observations made while searching the site’s surface were accomplished in September 2015. During our field research, the species Ruscus aculeatus L. was identified within the boundaries of the Natura 2000 ROSCI0240 Tăşad site and the 2.188 Tortorian limestones of the Tăşad reserve.
BOOK REVIEW </br> SIMION FLOREA MARIAN, BOTANICĂ POPORANĂ ROMÂNĂ; VOL. I – 699 PP; VOL. II- 711 PP; VOL III – 741 PP.
MILIAN GURĂU
ABOUT SIMION FLOREA MARIAN (1847-1907) He was born in Bukovina, at the time at which the province was under Austrian rule. Except for the first year, his classes were held in German. He had to go to schools in Ilişeşti (birth place), Suceava, Năsăud and Beiuş up to his 8th grade. He attended the Faculty of Orthodox Teology in Cernăuti. He described Romanian culture for 43 years using B. P. Haşdeu's ethnographic research method. Following his contribution to Romanian lexicography, he had been elected Academician at the age of 34. He left the Academy and the generations to come a total of 25 000 A4 written pages. He is acknowledged as the best Romanian folklorist. He has a long list of books and published works. We should mention that after he released the book Cromatica poporului roman, he received an award from the Romanian Academy and bought himself a big house in Suceava where he lived, currently housing which is now the museum „The Memorial and Documentary Fund S.F. Marian”. Out of all 520 articles in Botanica Poporana, he published only 54 during his lifetime, each article with its own bibliography. He managed to save from irreversible oblivion aspects of the Romanian culture which have been passed down throughout ages since the creation of the Romanian language up to the dawn of modern history.